Влияние ПЭГ-1500 на связывание дипиридамола с анионным каналом эритроцитов

• Main Authors: Ramazanov, V. V., Nardid, Ya. O., Olejnik, O. A., Bondarenko, V. A.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2003
• Subjects: еритроцити; аніонний канал; інгібітори транспорту; кріопротектори
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1073

The authors studied the effect of PEG-1500 on ability of dipyridamole to inhibit the H+ ions transport in erythrocytes. It was established that the inhibitor was not bound in anion channel of erythrocytes ghosts in sulfate medium, despite its ability to block the H+ ions entrance into cells. Using of fluoride loaded erythrocytes allowed to assume that dipyridamole’s inhibitory ability in cells placed to sulfate medium is controlled by chloride output flow during Jacobs-Stewart cycle functioning, and that proton transport does not reflect the whole amount of entering sulfate. Dipyridamole blockage of stated fraction of H+ ions transport was considerably withdrawn in PEG-1500 presense due to inhibitor’s redistribution from membrane to cryoprotectant solution.