Using EPR method there were investigated redox reactions of stable TEMPON nitroxyl radical with human placenta aqueoussaline extracts depending on placenta tissue storage temperature and extraction time. It was demonstrated, that with an increase in extraction time from 2 to 12 hrs, as well as place...

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Видавець:	Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
Дата:	2005
Автори:	Repina, S. V., Nardid, O. A., Rozanova, E. D., Tsymbal, L. V., Schetinsky, M. I., Naumenko, E. I.
Формат:	Стаття
Мова:	English
Опубліковано:	Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2005
Теми:	ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð»ÑŽÐ´Ð¸Ð½Ð¸ Ð•ÐŸÐ ÑÐ¿Ñ–Ð½Ð¾Ð²Ð¸Ñ… Ð·Ð¾Ð½Ð´Ñ–Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð¾Ð¶ÑƒÐ²Ð°Ð½Ð½Ñ Ð±Ñ–Ð»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¸Ð¹ ÑÐºÐ»Ð°Ð´ Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ñ– Ñ€ÐµÐ°ÐºÑ†Ñ–Ñ— human placenta extracts EPR of spine probes freezing protein-nucleotide composition redox reactions ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ‹ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ° ÐÐŸÐ ÑÐ¿Ð¸Ð½Ð¾Ð²Ñ‹Ñ… Ð·Ð¾Ð½Ð´Ð¾Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ñ‹Ð¹ ÑÐ¾ÑÑ‚Ð°Ð² Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ðµ Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¸
Онлайн доступ:	https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1207
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Problems of Cryobiology and Cryomedicine

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topic	ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð»ÑŽÐ´Ð¸Ð½Ð¸ Ð•ÐŸÐ ÑÐ¿Ñ–Ð½Ð¾Ð²Ð¸Ñ… Ð·Ð¾Ð½Ð´Ñ–Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð¾Ð¶ÑƒÐ²Ð°Ð½Ð½Ñ Ð±Ñ–Ð»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¸Ð¹ ÑÐºÐ»Ð°Ð´ Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ñ– Ñ€ÐµÐ°ÐºÑ†Ñ–Ñ— human placenta extracts EPR of spine probes freezing protein-nucleotide composition redox reactions ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ‹ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ° ÐÐŸÐ ÑÐ¿Ð¸Ð½Ð¾Ð²Ñ‹Ñ… Ð·Ð¾Ð½Ð´Ð¾Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ñ‹Ð¹ ÑÐ¾ÑÑ‚Ð°Ð² Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ðµ Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¸
spellingShingle	ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð»ÑŽÐ´Ð¸Ð½Ð¸ Ð•ÐŸÐ ÑÐ¿Ñ–Ð½Ð¾Ð²Ð¸Ñ… Ð·Ð¾Ð½Ð´Ñ–Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð¾Ð¶ÑƒÐ²Ð°Ð½Ð½Ñ Ð±Ñ–Ð»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¸Ð¹ ÑÐºÐ»Ð°Ð´ Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ñ– Ñ€ÐµÐ°ÐºÑ†Ñ–Ñ— human placenta extracts EPR of spine probes freezing protein-nucleotide composition redox reactions ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ‹ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ° ÐÐŸÐ ÑÐ¿Ð¸Ð½Ð¾Ð²Ñ‹Ñ… Ð·Ð¾Ð½Ð´Ð¾Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ñ‹Ð¹ ÑÐ¾ÑÑ‚Ð°Ð² Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ðµ Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¸ Repina, S. V. Nardid, O. A. Rozanova, E. D. Tsymbal, L. V. Schetinsky, M. I. Naumenko, E. I. ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
topic_facet	ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð»ÑŽÐ´Ð¸Ð½Ð¸ Ð•ÐŸÐ ÑÐ¿Ñ–Ð½Ð¾Ð²Ð¸Ñ… Ð·Ð¾Ð½Ð´Ñ–Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð¾Ð¶ÑƒÐ²Ð°Ð½Ð½Ñ Ð±Ñ–Ð»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¸Ð¹ ÑÐºÐ»Ð°Ð´ Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ñ– Ñ€ÐµÐ°ÐºÑ†Ñ–Ñ— human placenta extracts EPR of spine probes freezing protein-nucleotide composition redox reactions ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ‹ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ° ÐÐŸÐ ÑÐ¿Ð¸Ð½Ð¾Ð²Ñ‹Ñ… Ð·Ð¾Ð½Ð´Ð¾Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ñ‹Ð¹ ÑÐ¾ÑÑ‚Ð°Ð² Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ðµ Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¸
format	Article
author	Repina, S. V. Nardid, O. A. Rozanova, E. D. Tsymbal, L. V. Schetinsky, M. I. Naumenko, E. I.
author_facet	Repina, S. V. Nardid, O. A. Rozanova, E. D. Tsymbal, L. V. Schetinsky, M. I. Naumenko, E. I.
author_sort	Repina, S. V.
title	ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
title_short	ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
title_full	ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
title_fullStr	ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
title_full_unstemmed	ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
title_sort	ððÿð -ð¸ññð»ðµð´ð¾ð²ð°ð½ð¸ðµ ð¾ðºð¸ñð»ð¸ñ‚ðµð»ñœð½ð¾-ð²ð¾ñññ‚ð°ð½ð¾ð²ð¸ñ‚ðµð»ñœð½ñ‹ñ… ñ€ðµð°ðºñ†ð¸ð¹ ð²ð¾ð´ð½ð¾-ñð¾ð»ðµð²ñ‹ñ… ñðºññ‚ñ€ð°ðºñ‚ð¾ð² ð¿ð»ð°ñ†ðµð½ñ‚ñ‹ ñ‡ðµð»ð¾ð²ðµðºð°: ð²ð»ð¸ñð½ð¸ðµ ñ‚ðµð¼ð¿ðµñ€ð°ñ‚ñƒñ€ñ‹ ñ…ñ€ð°ð½ðµð½ð¸ñ ð¸ ð²ñ€ðµð¼ðµð½ð¸ ñðºññ‚ñ€ð°ð³ð¸ñ€ð¾ð²ð°ð½ð¸ñ
title_alt	EPR-Study of Redox Reactions of Human Placenta Aqueous-Saline Extracts: Storage Temperature and Extraction Time Effects ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ
description	Using EPR method there were investigated redox reactions of stable TEMPON nitroxyl radical with human placenta aqueoussaline extracts depending on placenta tissue storage temperature and extraction time. It was demonstrated, that with an increase in extraction time from 2 to 12 hrs, as well as placenta tissue storage at â€“20Â°C for a month, there was a decrease in redox process activity in extracts. Freezing of placenta tissue down to â€“196Â°C did not significantly affect redox activity of extracts in comparison with those from native placenta. Investigation results of protein-nucleotide composition in the obtained aqueous-saline extracts testify to the fact, that high-molecular extract fragments possess higher reductive activity in respect of TEMPON.Â
publisher	Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
publishDate	2005
url	https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1207
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first_indexed	2024-09-01T17:51:20Z
last_indexed	2024-09-19T09:19:05Z
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spelling	oai:ojs.pkp.sfu.ca:article-12072024-09-19T09:09:25Z EPR-Study of Redox Reactions of Human Placenta Aqueous-Saline Extracts: Storage Temperature and Extraction Time Effects ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ ÐÐŸÐ -Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ñ… Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¹ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ Repina, S. V. Nardid, O. A. Rozanova, E. D. Tsymbal, L. V. Schetinsky, M. I. Naumenko, E. I. ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð»ÑŽÐ´Ð¸Ð½Ð¸ Ð•ÐŸÐ ÑÐ¿Ñ–Ð½Ð¾Ð²Ð¸Ñ… Ð·Ð¾Ð½Ð´Ñ–Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð¾Ð¶ÑƒÐ²Ð°Ð½Ð½Ñ Ð±Ñ–Ð»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¸Ð¹ ÑÐºÐ»Ð°Ð´ Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ñ– Ñ€ÐµÐ°ÐºÑ†Ñ–Ñ— human placenta extracts EPR of spine probes freezing protein-nucleotide composition redox reactions ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ‹ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ° ÐÐŸÐ ÑÐ¿Ð¸Ð½Ð¾Ð²Ñ‹Ñ… Ð·Ð¾Ð½Ð´Ð¾Ð² Ð·Ð°Ð¼Ð¾Ñ€Ð°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ñ‹Ð¹ ÑÐ¾ÑÑ‚Ð°Ð² Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ñ‹Ðµ Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¸ Using EPR method there were investigated redox reactions of stable TEMPON nitroxyl radical with human placenta aqueoussaline extracts depending on placenta tissue storage temperature and extraction time. It was demonstrated, that with an increase in extraction time from 2 to 12 hrs, as well as placenta tissue storage at â€“20Â°C for a month, there was a decrease in redox process activity in extracts. Freezing of placenta tissue down to â€“196Â°C did not significantly affect redox activity of extracts in comparison with those from native placenta. Investigation results of protein-nucleotide composition in the obtained aqueous-saline extracts testify to the fact, that high-molecular extract fragments possess higher reductive activity in respect of TEMPON.Â ÐœÐµÑ‚Ð¾Ð´Ð¾Ð¼ ÐÐŸÐ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ Ñ€ÐµÐ°ÐºÑ†Ð¸Ð¸ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ñ-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÑÑ‚Ð°Ð±Ð¸Ð»ÑŒÐ½Ð¾Ð³Ð¾ Ð½Ð¸Ñ‚Ñ€Ð¾ÐºÑÐ¸Ð»ÑŒÐ½Ð¾Ð³Ð¾ Ñ€Ð°Ð´Ð¸ÐºÐ°Ð»Ð° Ð¢Ð•ÐœÐŸÐžÐ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ð¼Ð¸ ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð°Ð¼Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ñ‡ÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑ‚Ð¸ Ð¾Ñ‚ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ñ‹ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ñ Ñ‚ÐºÐ°Ð½Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ð¸ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ. ÐŸÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, Ñ‡Ñ‚Ð¾ ÑƒÐ²ÐµÐ»Ð¸Ñ‡ÐµÐ½Ð¸Ðµ Ð²Ñ€ÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑ‚Ñ€Ð°Ð³Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ Ñ 2-Ñ… Ð´Ð¾12-Ñ‚Ð¸ Ñ‡Ð°ÑÐ¾Ð², Ð° Ñ‚Ð°ÐºÐ¶Ðµ Ñ…Ñ€Ð°Ð½ÐµÐ½Ð¸Ðµ Ñ‚ÐºÐ°Ð½Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ð¿Ñ€Ð¸ â€“20Â°Ð¡ Ð² Ñ‚ÐµÑ‡ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÑÑ†Ð° Ð¿Ñ€Ð¸Ð²Ð¾Ð´Ð¸Ñ‚ Ðº ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸ÑŽ Ð°ÐºÑ‚Ð¸Ð²Ð½Ð¾ÑÑ‚Ð¸ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾Ð³Ð¾ Ð¿Ñ€Ð¾Ñ†ÐµÑÑÐ° Ð² ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð°Ñ…. Ð—Ð°Ð¼Ð¾Ñ€Ð°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ Ñ‚ÐºÐ°Ð½Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹ Ð´Ð¾ â€“196Â°Ð¡ Ð½Ðµ Ð²Ñ‹Ð·Ñ‹Ð²Ð°Ð»Ð¾ ÑÑƒÑ‰ÐµÑÑ‚Ð²ÐµÐ½Ð½Ñ‹Ñ… Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¾ÐºÐ¸ÑÐ»Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾-Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾Ð¹ Ð°ÐºÑ‚Ð¸Ð²Ð½Ð¾ÑÑ‚Ð¸ ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² Ð¿Ð¾ ÑÑ€Ð°Ð²Ð½ÐµÐ½Ð¸ÑŽ Ñ ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð°Ð¼Ð¸ Ð¸Ð· Ð½Ð°Ñ‚Ð¸Ð²Ð½Ð¾Ð¹ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ñ‹. Ð ÐµÐ·ÑƒÐ»ÑŒÑ‚Ð°Ñ‚Ñ‹ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð±ÐµÐ»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¾Ð³Ð¾ ÑÐ¾ÑÑ‚Ð°Ð²Ð° Ð¿Ð¾Ð»ÑƒÑ‡Ð°ÐµÐ¼Ñ‹Ñ… Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÐµÐ²Ñ‹Ñ… ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð² ÑÐ²Ð¸Ð´ÐµÑ‚ÐµÐ»ÑŒÑÑ‚Ð²ÑƒÑŽÑ‚ Ð¾ Ñ‚Ð¾Ð¼, Ñ‡Ñ‚Ð¾ Ð±Ð¾Ð»ÐµÐµ Ð²Ñ‹ÑÐ¾ÐºÐ¾Ð¹ Ð²Ð¾ÑÑÑ‚Ð°Ð½Ð¾Ð²Ð¸Ñ‚ÐµÐ»ÑŒÐ½Ð¾Ð¹ Ð°ÐºÑ‚Ð¸Ð²Ð½Ð¾ÑÑ‚ÑŒÑŽ Ð¿Ð¾ Ð¾Ñ‚Ð½Ð¾ÑˆÐµÐ½Ð¸ÑŽ Ðº Ð¢Ð•ÐœÐŸÐžÐÑƒ Ð¾Ð±Ð»Ð°Ð´Ð°ÑŽÑ‚ Ð²Ñ‹ÑÐ¾ÐºÐ¾Ð¼Ð¾Ð»ÐµÐºÑƒÐ»ÑÑ€Ð½Ñ‹Ðµ Ñ„Ñ€Ð°Ð³Ð¼ÐµÐ½Ñ‚Ñ‹ ÑÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð¾Ð². ÐœÐµÑ‚Ð¾Ð´Ð¾Ð¼ Ð•ÐŸÐ Ð´Ð¾ÑÐ»Ñ–Ð´Ð¶ÑƒÐ²Ð°Ð»Ð¸ Ñ€ÐµÐ°ÐºÑ†Ñ–Ñ— Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð½Ñ-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð»ÐµÐ½Ð½Ñ ÑÑ‚Ð°Ð±Ñ–Ð»ÑŒÐ½Ð¾Ð³Ð¾ Ð½Ñ–Ñ‚Ñ€Ð¾ÐºÑÐ¸Ð»ÑŒÐ½Ð¾Ð³Ð¾ Ñ€Ð°Ð´Ñ–ÐºÐ°Ð»Ð° Ð¢Ð•ÐœÐŸÐžÐ Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÑŒÐ¾Ð²Ð¸Ð¼Ð¸ ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð°Ð¼Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð»ÑŽÐ´Ð¸Ð½Ð¸ Ð² Ð·Ð°Ð»ÐµÐ¶Ð½Ð¾ÑÑ‚Ñ– Ð²Ñ–Ð´ Ñ‚ÐµÐ¼Ð¿ÐµÑ€Ð°Ñ‚ÑƒÑ€Ð¸ Ð·Ð±ÐµÑ€ÐµÐ¶ÐµÐ½Ð½Ñ Ñ‚ÐºÐ°Ð½Ð¸Ð½Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ñ– Ñ‡Ð°ÑÑƒ ÐµÐºÑÑ‚Ñ€Ð°Ð³ÑƒÐ²Ð°Ð½Ð½Ñ. ÐŸÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, Ñ‰Ð¾ Ð·Ð±Ñ–Ð»ÑŒÑˆÐµÐ½Ð½Ñ Ñ‡Ð°ÑÑƒ ÐµÐºÑÑ‚Ñ€Ð°Ð³ÑƒÐ²Ð°Ð½Ð½Ñ Ð· 2-Ñ… Ð´Ð¾ 12-Ñ‚Ð¸ Ð³Ð¾Ð´Ð¸Ð½, Ð° Ñ‚Ð°ÐºÐ¾Ð¶ Ð·Ð±ÐµÑ€ÐµÐ¶ÐµÐ½Ð½Ñ Ñ‚ÐºÐ°Ð½Ð¸Ð½Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð¿Ñ€Ð¸ â€“20Â°Ð¡ Ð¿Ñ€Ð¾Ñ‚ÑÐ³Ð¾Ð¼ Ð¼Ñ–ÑÑÑ†Ñ Ð¿Ñ€Ð¸Ð·Ð²Ð¾Ð´Ð¸Ñ‚ÑŒ Ð´Ð¾ Ð·Ð½Ð¸Ð¶ÐµÐ½Ð½Ñ Ð°ÐºÑ‚Ð¸Ð²Ð½Ð¾ÑÑ‚Ñ– Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¿Ñ€Ð¾Ñ†ÐµÑÑƒ Ð² ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð°Ñ…. Ð—Ð°Ð¼Ð¾Ñ€Ð¾Ð¶ÑƒÐ²Ð°Ð½Ð½Ñ Ñ‚ÐºÐ°Ð½Ð¸Ð½Ð¸ Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸ Ð´Ð¾ â€“196Â°Ð¡ Ð½Ðµ Ð²Ð¸ÐºÐ»Ð¸ÐºÐ°Ñ” ÑÑƒÑ‚Ñ‚Ñ”Ð²Ð¸Ñ… Ð·Ð¼Ñ–Ð½ Ð¾ÐºÐ¸ÑÐ»ÑŽÐ²Ð°Ð»ÑŒÐ½Ð¾-Ð²Ñ–Ð´Ð½Ð¾Ð²Ð½Ð¾Ñ— Ð°ÐºÑ‚Ð¸Ð²Ð½Ð¾ÑÑ‚Ñ– ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ–Ð² Ñƒ Ð¿Ð¾Ñ€Ñ–Ð²Ð½Ð½ÑÐ½Ð½Ñ– Ð· ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ð°Ð¼Ð¸ Ð· Ð½Ð°Ñ‚Ð¸Ð²Ð½Ð¾Ñ— Ð¿Ð»Ð°Ñ†ÐµÐ½Ñ‚Ð¸. Ð ÐµÐ·ÑƒÐ»ÑŒÑ‚Ð°Ñ‚Ð¸ Ð´Ð¾ÑÐ»Ñ–Ð´Ð¶ÐµÐ½ÑŒ Ð±Ñ–Ð»Ð¾Ðº-Ð½ÑƒÐºÐ»ÐµÐ¾Ñ‚Ð¸Ð´Ð½Ð¾Ð³Ð¾ ÑÐºÐ»Ð°Ð´Ñƒ Ð¾Ð´ÐµÑ€Ð¶Ð°Ð½Ð¸Ñ… Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¾Ð»ÑŒÐ¾Ð²Ð¸Ñ… ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ–Ð² ÑÐ²Ñ–Ð´Ñ‡Ð°Ñ‚ÑŒ Ð¿Ñ€Ð¾ Ñ‚Ðµ, Ñ‰Ð¾ Ð±Ñ–Ð»ÑŒÑˆ Ð²Ð¸ÑÐ¾ÐºÑƒ Ð²Ñ–Ð´Ð½Ð¾Ð²Ð»ÑŽÑŽÑ‡Ñƒ Ð°ÐºÑ‚Ð¸Ð²Ð½Ñ–ÑÑ‚ÑŒ Ð²Ñ–Ð´Ð½Ð¾ÑÐ½Ð¾ Ð¢Ð•ÐœÐŸÐžÐÑƒ Ð¼Ð°ÑŽÑ‚ÑŒ Ð²Ð¸ÑÐ¾ÐºÐ¾Ð¼Ð¾Ð»ÐµÐºÑƒÐ»ÑÑ€Ð½Ñ– Ñ„Ñ€Ð°Ð³Ð¼ÐµÐ½Ñ‚Ð¸ ÐµÐºÑÑ‚Ñ€Ð°ÐºÑ‚Ñ–Ð². Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2005-12-25 Article Article Research article application/pdf https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1207 Problems of Cryobiology and Cryomedicine; Vol. 15 No. 4 (2005): Problems of Cryobiology; 591-598 Проблемы криобиологии и криомедицины; Том 15 № 4 (2005): Problems of Cryobiology; 591-598 Проблеми кріобіології і кріомедицини; Том 15 № 4 (2005): Problems of Cryobiology; 591-598 2518-7376 2307-6143 en https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1207/1289

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