Определение метаболической активности свежевыделенных и криоконсервированных клеток эмбриональной печени человека с помощьюAlamar Blue-теста

• Date: 2005
• Main Authors: Petrenko, Yu. A., Gorokhova, N. A., Sandomirsky, B. P., Petrenko, A. Yu.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2005
• Subjects: Alamar Blue; клітини ембріональної печінки; кріоконсервування; метаболiчна активність; флуоресценція
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1208
• Journal Title: Problems of Cryobiology and Cryomedicine

The possibility of using the metabolic indicator Alamar Blue (AB) for fetal liver (FL) cells viability and proliferative activity assessment before and after cryopreservation was investigated. It is shown, that AB is the integral indicator of cells metabolic activity, which reflects the plasma membrane integrity as well as glycolysis intensity and respiratory chain activity in mitochondria. The inhibitory analysis of the AB reduction by human FL cells demonstrated that the glycolysis took the significant part in the total metabolic activity of cells of embryonic origin. The results demonstrate that the AB is a highly sensitive viability test for human FL cells and it could be used for the screening of cryopreservation protocol efficiency.