Влияние компонентов криозащитных сред на жизнеспособность сперматозоидов собак в процессе криоконсервирования

Влияние компонентов криозащитных сред на жизнеспособность сперматозоидов собак в процессе криоконсервирования

There was studied the effect of such cryoprotectants (CP) as: glycerol (GL), ethylene glycol (EG), dimethyl sulfoxide (DMSO), N-N-dimethyl formamide (DMF), 1,2-propanediol (1,2-PD) in 1 M concentration on dog spermatozoa (DS) viability before and after freeze-thawing in 3 media, differing by composi...

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Bibliographische Detailangaben
Datum:2005
Hauptverfasser: Linnik, T. P., Egorov, M. I., Dyubko, T. S., Belonozhko, A. P.
Format: Artikel
Sprache:English
Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2005
Schlagworte:
cперматозоїди
рухливість
кріоконсервування
кріопротектори
альбумін
яєчний жовток
Online Zugang:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1214
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Назва журналу:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
Beschreibung
Zusammenfassung:There was studied the effect of such cryoprotectants (CP) as: glycerol (GL), ethylene glycol (EG), dimethyl sulfoxide (DMSO), N-N-dimethyl formamide (DMF), 1,2-propanediol (1,2-PD) in 1 M concentration on dog spermatozoa (DS) viability before and after freeze-thawing in 3 media, differing by composition. The first medium (TFY) comprised the yolk of chicken eggs, the second one (ТFA) consisted of egg albumin, the third one (ТF) was used without additives. It was established, that the highest viability of DS both before and after cryopreservation was observed under DMF protection in TFA medium. A possible mechanism of egg albumin effect on DS viability in combination with DMF is discussed.

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