Влияние инкубации с криопротекторами на количество эпитопов Gal-α-1,3-Gal в клетках линии PK-15

• Main Authors: Bohuslavskyi, Konstantin I., Alabedalkarim, Natalia M., Pakhomov, Alexsandr V., Bozhok, Galyna A.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2016
• Subjects: епітоп Gal-α-1; 3-Gal; кріопротектори; диметилсульфоксид; поліетиленгліколь
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1242

Transplantation of bioprostheses derived from bovine and porcine tissues is an advanced method of correcting defects in blood vessels and valves, however when using it a hyperacute immune response associated with the presence of Gal-alpha- 1,3-Gal (α-Gal epitope) on the surface of cell membranes can develop. We have studied the effect of penetrating and nonpenetrating cryoprotectants on the amount of α-Gal epitopes on membranes of PK-15 cell line of porcine origin. Cell labeling with BSIB4 lectin conjugated with FITC and flow cytometry revealed a significant increase in the number of fluorescent cells after incubation with various concentrations of dimethyl sulfoxide and polyethylene glycol with MW of 1500 (PEG-1500). The revealed effect may be associated with an additional exposure of α-Gal epitopes on cell membrane, locally deformed due to the action of cryoprotectants.Probl Cryobiol Cryomed 2016; 26(4): 331–339