Криоконсервирование мультиклеточных сфероидов, полученных из надпочечников новорожденных поросят

• Дата: 2017
• Автори: Plaksina, Ekaterina M., Sidorenko, Olga C., Bozhok, Galina A.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2017
• Теми: льтиклітинні сфероїди; наднирники; диметилсульфоксид; β-III-тубулін; нейробластоподібні клітини; фібробластоподібні клітини; мультиклеточные сфероиды; надпочечники; β-III-тубулин; нейробластоподобные клетки; фибробластоподобные клетки; новорожденные поросята.; multicellular spheroids; adrenal glands; dimethylsulfoxide; β-III-tubulin; neuroblast-like cells; fibroblast-like cells; rats
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1353
• Назва журналу: Problems of Cryobiology and Cryomedicine

Application of special in vitro culture techniques for the cells, derived from different animal and human organs, makespossible the obtaining of multicellular spheroids (MSs), being the natural 3-D environment for cells unlike the standard culture with thecells in monolayer. Previously we have shown that MSs formed in the newborn piglet adrenal cell culture are capable to produce theneuroblast-like cells, expressing the neuronal marker β-III-tubulin. In the present work we have assessed the regimens for MSscryopreservation using 5, 7 and 10% dimethyl sulfoxide (DMSO) and 25% fetal bovine serum (FBS). Analysis of some characteristicsof cryopreserved MSs (surface adhesion, capability to produce the neuroblast-like cells and monolayer formation by fibroblast-likecells) allowed to choose the cryopreservation regimen with 1°C/min cooling rate in the presence of 10% DMSO as the most optimalone. The FBS supplement to the medium did not significantly affect the cryopreservation outcome, although there was found atendency to increase the capability of cryopreserved MSs to produce the neuroblast- and fibroblast-like cells.Probl Cryobiol Cryomed 2017; 27(4): 322-333