Эффективность комбинированных криоконсервантов, содержащих глицерин или 1,2-пропандиол, при замораживании эритроцитов

• Date: 2011
• Main Author: Ramazanov, V. V.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2011
• Subjects: еритроцити; комбіновані кріоконсерванти; поток іонів H; осмотичний гемоліз; поток іонів H
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/138
• Journal Title: Problems of Cryobiology and Cryomedicine

The osmotic properties of erythrocytes frozen-thawed in combined cryopreservatives, containing polymeric non-penetrating (dextran, polyethylene glycol) and penetrating (glycerol, 1,2-propane diol) cryoprotectants were studied. It was established that in erythrocytes frozen-thawed in the presence of polymers the increasing of H+ ion flow rate and osmotic fragility in the environment with 0.45–0.9% NaCl was observed. It is sufficient to add 5% glycerol or 1,2-propane diol (1,2-PD) to the medium to preserve osmotic properties of frozen-thawed cells. The obtained results enable to suggest that cryoprotective efficiency of combined cryopreservatives, containing non-penetrating and penetrating cryoprotectants is determined both by the contribution of cryoprotective components differing by action mechanism into the sum protection efficiency during freezing-thawing and by the weakening of posthypertonic stress in cells during freeze-thawing.