Обеспечение условий сопоставимости результатов криоконсервирования эмбрионов млекопитающих

There was proposed mathematical model of estimation of initial state and efficiency of cryopreservation of mammalian embryos, providing the comparability of the results when using different methods of freezing. It has been shown that initial viability of bovine embryos makes 70–100% and depends on...

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Bibliographische Detailangaben
Datum:2011
Hauptverfasser: Gorbunov, L. V., Gordienko, E. A.
Format: Artikel
Sprache:English
Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2011
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Online Zugang:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/141
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Назва журналу:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
Beschreibung
Zusammenfassung:There was proposed mathematical model of estimation of initial state and efficiency of cryopreservation of mammalian embryos, providing the comparability of the results when using different methods of freezing. It has been shown that initial viability of bovine embryos makes 70–100% and depends on physiological state of embryos and their donor. Cryopreservation efficiency of murine and bovine embryos varies from 53 to 95%, which is stipulated by the state of embryo and peculiarities of the methods applied. Taking into account of biological object features and the cryopreservation way (initial state and efficiency of stages) provides the statistical significance of the obtained values P ≥ 0.95 with the decrease in the number of mammalian embryos 10 times.