Електрична провідність і стійкість мембран ооцитів миші до дії імпульсного електричного поля в розчинах кріопротекторів

• Date: 2019
• Main Authors: Smolyaninova, Yevgeniya I., Shigimaga, Viktor O., Kolesnikova, Alla O., Popivnenko, Lyudmila I., Todrin, Aleksandr F.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2019
• Subjects: ооцити миші; плазматична мембрана; електрична провідність; кріопротектор; електропорація; електричний пробій; імпульсне електричне поле; напруженість
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1458
• Journal Title: Problems of Cryobiology and Cryomedicine

Using the method of pulsed conductometry, the values of electric conductivity of mouse oocytes in 1.0 M solutions of penetrating cryoprotectants have been first determined and the stability of their plasma membranes to the effect of pulsed electric field of increasing intensity has been investigated. For the oocytes, incubated in ethylene glycol (EG), 1,2-propanediol (1,2-PD), 2,3-butanediol (2,3-BD) and dimethyl sulfoxide (DMSO) solutions, the electric conductivity values were (23.6 ± 3.4), (21.3 ± 5.8) and (21.0 ± 2.3) and (23.6 ± 7.1) μS/cm respectively. The values of electric conductivity for mouse oocytes in acetamide (AC) and formamide (FA) solutions were (16.5 ± 6.1) and (16.9 ±± 10.7) μS/cm, respectively. Cryoprotectant solutions, belonging to alcohols (EG, 2,3-BD), as well as DMSO, were shown to have a stabilizing effect on the mouse oocyte plasma membranes under electric field action. The AC and FA solutions caused no stabilizing effect on mouse oocyte membranes, which was manifested in development of irreversible electric breakdown of plasma membranes with increasing electric field intensity. Probl Cryobiol Cryomed 2018; 28(4): 311-321