Вплив проникаючих кріопротекторів на мембранний Ñкелет еритроцитів
The impact of formamide (FA), N-methylformamide (MFA), N,N-dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) on the spectrin-based membrane skeleton of human erythrocytes was studied by thermal dielectroscopy. Upon heating the erythrocyte suspension, spectrin denatures at 49.5°C causing suspens...
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| Date: | 2019 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2019
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| Subjects: | |
| Online Access: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1550 |
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| Journal Title: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and Cryomedicine| Summary: | The impact of formamide (FA), N-methylformamide (MFA), N,N-dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) on the spectrin-based membrane skeleton of human erythrocytes was studied by thermal dielectroscopy. Upon heating the erythrocyte suspension, spectrin denatures at 49.5°C causing suspension impedance to change by ΔZ* = ΔZ' + jΔZ". The - ΔZ" vs ΔZ' plot reveals two dielectric relaxations on spectrin, called beta (0.05-1.0 MHz) and gamma (1.0-10 MHz) relaxations. At concentrations up to 5 M (relevant to cryoprotection) DMF and DMSO specifically subdued gamma-relaxation, while FA and MFA specifically inhibited beta-relaxation. Each effect was eliminated by preliminary washing of cryoprotectant and presumably did not depend on the osmotic pressure produced. Applied in combination, DMSO and FA neutralized their effects. Based on previous study, the results indicate that tested cryoprotectants detached the membrane skeleton from the lipid membrane, thereby compromising cell integrity. Probl Cryobiol Cryomed 2019; 29(3): 237–245. |
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