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Гістологічне дослідження нативних і кріоконсервованих неонатальних сім’яників щурів після інтратестікулярної алотрансплантації
Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals. Moreover, the transplantation of testicular tissue is involved into investigation of the inborn and acquired defects of testicular t...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2020
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| Subjects: | |
| Online Access: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1600 |
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| Summary: | Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals. Moreover, the transplantation of testicular tissue is involved into investigation of the inborn and acquired defects of testicular tissue. Thus the improvement of cryopreservation and transplantation methods is of great interest. Here, we have compared the findings of histological examination of allotransplanted native and cryopreserved neonatal testis. It has been shown that the usage of DMEM/F12 supplemented with 5 mg/ml bovine serum albumin, 0.05 M sucrose, 20 mM Hepes, 1.5 M dimethylsulfoxide as a cryoprotectant, controlled cooling, preservation at -196°C, water bath warming, followed by intratesticular allotransplantation of neonatal rat testis results in the development of testicular interstitium and spermatogenic epithelium of donor organs into recipient body.
Probl Cryobiol Cryomed 2020; 30(1): 34-46 |
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