Збереженість клітин інтерстицію сім’яника щурів після кріоконсервування у середовищах на основі гідроксиетил крохмалю

• Date: 2021
• Main Author: Pakhomov , Oleksandr
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2021
• Subjects: кріоконсервування; кріопротектори; клітини Лейдига; гідроксиетил крохмаль
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1715
• Journal Title: Problems of Cryobiology and Cryomedicine

The development of cryoprotective serum-free or xeno-free media is required for safe use of cryopreserved testicular material for transplantation. In this study, the solutions containing 10% fetal bovine serum (FBS) or 5 mg/ml bovine serum albumin (BSA) did not significantly enhance the general survival of interstitial cells (ICs) after cryopreservation but increase their metabolic activity and steroid producing cell (HSD+-cells) survival. The use of 50 and 100 mg/ml hydroxyethyl starch (HES) in DMSO-based cryoprotective solutions instead of BSA or FBS enabled the improvement of the IC general survival and the survival of HSD+-cells. The use of HES supplemented media allowed to decrease the dimethyl sulfoxide (DMSO) concentration from 1.4 to 0.7 M and to preserve the amount and metabolic activity of ICs. Thus, designing cryoprotective media containing DMSO and HES can facilitate the formulation of serum-free solutions for cryopreservation that in turn paves a way for implementation of the use of cryopreserved material for practical medicine. Probl Cryobiol Cryomed 2021; 31(1): 070–081