Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку
To date, the number of women giving birth to their first child in late reproductive age is increasing around the world. This stipulates a need in designing the new approaches to restore the uterine contractile activity. In this paper, the histological features of uterus and its contractile activity...
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| Datum: | 2021 |
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Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2021
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| Назва журналу: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and Cryomedicine| _version_ | 1859479314107465728 |
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| author | Nesteruk, Hanna Ustichenko , Viktoriya Alabedalkarim , Nataliya Padalko , Volodymyr Protsenko , Olena Legach , Ievgen |
| author_facet | Nesteruk, Hanna Ustichenko , Viktoriya Alabedalkarim , Nataliya Padalko , Volodymyr Protsenko , Olena Legach , Ievgen |
| author_sort | Nesteruk, Hanna |
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| collection | OJS |
| datestamp_date | 2021-11-11T08:31:32Z |
| description | To date, the number of women giving birth to their first child in late reproductive age is increasing around the world. This stipulates a need in designing the new approaches to restore the uterine contractile activity. In this paper, the histological features of uterus and its contractile activity have been experimentally studied in differently aged rats after the dorsal root ganglia cryoextract (DRGCE) administration. The cryoextract was derived from dorsal root ganglia of neonatal piglets by three-fold freezing down to –196°C in saline, followed by thawing at room temperature, homogenization and centrifugation. Here, we used the female rats of reproductive age (RA, 6-month-old) and those of late reproductive age (LRA, 14-month-old). Animals of both age groups received intraperitoneally either DRGCE (experimental groups) or saline (control groups) for 9 days by 0.2 ml. To days 28–29 after administration beginning the animals were sacrificed and the uterine fragments were taken for histological examination and study of oxytocin (OT)-induced uterine contractile activity (UCA). The strength of OT-induced uterine isometric contraction was found to decrease by 28.6% in LRA rats vs. the RA ones. The DRGCE administration to LRA rats increased the UCA indices, namely the contractile frequency, contractile amplitude and strength of isometric contraction augmented by 25, 9.8 and 30% respectively, as compared with the group of the same age without DRGCE introduction. This effect was observed on the background normal uterine histological structure and unchanged myometrial thickness.
 
Probl Cryobiol Cryomed 2021; 31(3): 258–267 |
| first_indexed | 2025-09-24T17:25:42Z |
| format | Article |
| id | oai:ojs.pkp.sfu.ca:article-1744 |
| institution | Problems of Cryobiology and Cryomedicine |
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| language | English |
| last_indexed | 2026-03-12T17:47:41Z |
| publishDate | 2021 |
| publisher | Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine |
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| spelling | oai:ojs.pkp.sfu.ca:article-17442021-11-11T08:31:32Z Impact of Dorsal Root Ganglia Cryoextract on Histological Steatures in Dices and Contractility of Uterus in Differently Aged Rats Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку Nesteruk, Hanna Ustichenko , Viktoriya Alabedalkarim , Nataliya Padalko , Volodymyr Protsenko , Olena Legach , Ievgen cryoextract dorsal root ganglia neurotrophic factors uterus contractile activity кріоекстракт спінальні ганглії нейротрофічні фактори матка скорочувальна активність To date, the number of women giving birth to their first child in late reproductive age is increasing around the world. This stipulates a need in designing the new approaches to restore the uterine contractile activity. In this paper, the histological features of uterus and its contractile activity have been experimentally studied in differently aged rats after the dorsal root ganglia cryoextract (DRGCE) administration. The cryoextract was derived from dorsal root ganglia of neonatal piglets by three-fold freezing down to –196°C in saline, followed by thawing at room temperature, homogenization and centrifugation. Here, we used the female rats of reproductive age (RA, 6-month-old) and those of late reproductive age (LRA, 14-month-old). Animals of both age groups received intraperitoneally either DRGCE (experimental groups) or saline (control groups) for 9 days by 0.2 ml. To days 28–29 after administration beginning the animals were sacrificed and the uterine fragments were taken for histological examination and study of oxytocin (OT)-induced uterine contractile activity (UCA). The strength of OT-induced uterine isometric contraction was found to decrease by 28.6% in LRA rats vs. the RA ones. The DRGCE administration to LRA rats increased the UCA indices, namely the contractile frequency, contractile amplitude and strength of isometric contraction augmented by 25, 9.8 and 30% respectively, as compared with the group of the same age without DRGCE introduction. This effect was observed on the background normal uterine histological structure and unchanged myometrial thickness.   Probl Cryobiol Cryomed 2021; 31(3): 258–267 Probl Cryobiol Cryomed 2021; 31(3): 258–267 To date, the number of women giving birth to their first child in late reproductive age is increasing around the world. This stipulates a need in designing the new approaches to restore the uterine contractile activity. In this paper, the histological features of uterus and its contractile activity have been experimentally studied in differently aged rats after the dorsal root ganglia cryoextract (DRGCE) administration. The cryoextract was derived from dorsal root ganglia of neonatal piglets by three-fold freezing down to –196°C in saline, followed by thawing at room temperature, homogenization and centrifugation. Here, we used the female rats of reproductive age (RA, 6-month-old) and those of late reproductive age (LRA, 14-month-old). Animals of both age groups received intraperitoneally either DRGCE (experimental groups) or saline (control groups) for 9 days by 0.2 ml. To days 28–29 after administration beginning the animals were sacrificed and the uterine fragments were taken for histological examination and study of oxytocin (OT)-induced uterine contractile activity (UCA). The strength of OT-induced uterine isometric contraction was found to decrease by 28.6% in LRA rats vs. the RA ones. The DRGCE administration to LRA rats increased the UCA indices, namely the contractile frequency, contractile amplitude and strength of isometric contraction augmented by 25, 9.8 and 30% respectively, as compared with the group of the same age without DRGCE introduction. This effect was observed on the background normal uterine histological structure and unchanged myometrial thickness.   Probl Cryobiol Cryomed 2021; 31(3): 258–267 Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2021-10-08 Article Article application/pdf https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1744 10.15407/cryo31.03.258 Problems of Cryobiology and Cryomedicine; Vol. 31 No. 3 (2021): Probl Cryobiol Cryomed; 258–267 Проблемы криобиологии и криомедицины; Том 31 № 3 (2021): Probl Cryobiol Cryomed; 258–267 Проблеми кріобіології і кріомедицини; Том 31 № 3 (2021): Probl Cryobiol Cryomed; 258–267 2518-7376 2307-6143 en https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1744/1849 https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | кріоекстракт спінальні ганглії нейротрофічні фактори матка скорочувальна активність Nesteruk, Hanna Ustichenko , Viktoriya Alabedalkarim , Nataliya Padalko , Volodymyr Protsenko , Olena Legach , Ievgen Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| title | Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| title_alt | Impact of Dorsal Root Ganglia Cryoextract on Histological Steatures in Dices and Contractility of Uterus in Differently Aged Rats |
| title_full | Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| title_fullStr | Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| title_full_unstemmed | Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| title_short | Вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| title_sort | вплив кріоекстракту спінальних гангліїв на гістологічні характеристики та скорочувальну активність матки щурів різного віку |
| topic | кріоекстракт спінальні ганглії нейротрофічні фактори матка скорочувальна активність |
| topic_facet | cryoextract dorsal root ganglia neurotrophic factors uterus contractile activity кріоекстракт спінальні ганглії нейротрофічні фактори матка скорочувальна активність |
| url | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1744 |
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