Осмотичні характеристики еритроцитів після заморожування у модифікованих за складом кріоконсервантах
Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the eryth...
Збережено в:
| Дата: | 2022 |
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| Автори: | , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2022
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| Теми: | |
| Онлайн доступ: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1791 |
| Теги: |
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| Назва журналу: | Problems of Cryobiology and Cryomedicine |
Репозитарії
Problems of Cryobiology and Cryomedicine| Резюме: | Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the erythrocyte characteristics at different stages of freezing and hypothermic storage. Reduction of 1,2-propanediol and NaCl concentrations when augmenting the sucrose content in cryopreservative agent enabled changing a two-stage mode of erythrocyte freeze-thawing to the single-stage one. The presented here modification of cryoprotective medium allowed to diminish the level of damage to erythrocytes during freeze-thawing-washing out, that might prevent the inflammation development in a body when transfusing these cells.
Probl Cryobiol Cryomed 2022; 32(2):092–103 |
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