Склад та біологічна активність кріоекстрактів із тканин фетоплацентарного походження за різних умов виготовлення

• Дата: 2023
• Автори: Repin, Mykola, Chizh, Yuliia, Marchenko, Larysa, Govorukha, Tetyana, Narozhnyy, Stanislav
• Формат: Стаття
• Мова: Англійська
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2023
• Теми: кріоекстракт плаценти; кріоекстракт фетальних тканин; гель-проникна хроматографія; фагоцитарна активність нейтрофілів
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1863
• Назва журналу: Problems of Cryobiology and Cryomedicine

The protein-peptide composition of fetal tissues (FTCEs) and placenta (PCE) cryoextracts of rats was investigated by gel permeation chromatography. Cryoextracts were derived from tissue homogenates using the freeze-warming modes: 1 – single (–20°С); 2 – double (–20; –196°С) and 3 – triple (–20; –196; –196°С) ones. The biological activity of cryoextracts was in vitro evaluated by the phagocytic activity of neutrophil granulocytes (NG) of the blood of intact rats after incubation with an inactivated culture of Staphylococcus aureus (2 × 109 cells/ml) for 45 and 120 min for the concentrations of extracts in the incubation medium: 1.6; 3.1; 6 mg/ml in terms of dry substance. The use of mode 3 ensured a greater yield of total protein and low molecular weight compounds of a protein-peptide nature in the composition of cryoextracts. The content of low-molecular-weight fractions in FTCE exceeded that in PCE, regardless of the obtaining mode. Incubation of NG with cryoextracts did not signifi cantly increase the number of neutrophils which entered into phagocytosis. However, all the samples of PCE and FTCE were characterized by a dose-dependent rise in the NG absorption activity during the 45-minute incubation compared to the control. Probl Cryobiol Cryomed 2023; 33(1):003–013