Взаимодействие криопротекторов с липосомами из суммарных липидов спермиев петуха

• Main Authors: Linnik, T. P., Dyubko, T. S., Martynyuk, I. N., Tereschenko, A. V.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2010
• Subjects: півень; сперматозоїди; ліпіди; ліпосоми; кріопротектори; флуоресцентний зонд
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/215

Using the fluorescent probe FME we studied interactions of the cryoprotectants: ethylene glycol (EG), 1,2-propane diol (1,2-PD), N,N-dimethyl formamide (DMFA), N,N-dimethyl acetamide (DMAc), glycerol and dimethyl sulfoxide (DMSO) with liposomes made from the fowl spermatozoa total lipid (FSTL). It was established that the level of CP impacts on bilayer is determined by hydrophilic-hydrophobic balance of their molecules and showed a concentration dependence. The cryoprotectants are ranked by their increasing influence on FME fluorescence spectral parameters in RSTL liposomes as follows: glycerol ≤ EG < 1,2-PD < DMSO < DMAc ≤ DMFA, that corresponds the growth in their distribution coefficients in the system “water – non-polar phase”. A mechanism of the cryoprotectant impacts on bonds forming bilayer is discussed.