Воспроизводимость результатов криоконсервирования черенков различных сортов семечковых плодовых деревьев

• Дата: 2009
• Автор: Gorbunov, L. V.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2009
• Теми: apple and pear cuttings; cryopreservation; viability; efficiency; cooling and thawing rates; живці яблуні Ñ– груші; кріоконсервування; життєздатність; ефективність; швидкості охолодження Ñ– відігрівання; черенки яблони и груши; криоконсервирование; жизнеспособность; эффективность; скорости охлаждения и отогрева
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/236
• Назва журналу: Problems of Cryobiology and Cryomedicine

Viable frozen-thawed samples of apple (Belyy Naliv, Teremok, Radost), pear (Velyka Litnya, Ulyublena Klapa, Vdala, Zelena Mliivska) were obtained. With dispersion analysis it has been established that the development probability of frozen-thawed plant cuttings depends on variety and cryopreservation method. Mean indices of viability for different apple varieties differ by 32% and pear by 86%. Maximal indices of cuttings’ viability are obtained after their temperature adaptation at -10°C during 14ч60 days, stepwise cooling of the samples with 0.1–0.5°C/hr rate down to -30°C (exposure 3–7 days), direct plunging into liquid nitrogen, storage from 1 to 30 days and thawing rate of 70°C/min.