Защитный эффект метилцеллюлозы при криоконсервировании перевиваемых клеток в бессывороточной среде

• Datum: 2013
• Hauptverfasser: Koschiy, Svetlana V., Goltsev, Anatoliy N., Vysekantsev, Igor P., Martsenyuk, Valentina F., Gurina, Tatyana M., Sokol, Larisa V.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
• Schlagworte: клітини; які перевиваються; кріоконсервування; захисне середовище; метилцелюлоза
• Online Zugang: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/28
• Назва журналу: Problems of Cryobiology and Cryomedicine

There was experimentally shown a possibility to replace blood serum for methylcellulose (MC) in the media for cryopreservation of inoculated cells. Combination of 0.1% MC with 5% dimethyl sufoxide (DMSO) in the preservation media provided the highest indices of post-thaw cell survival. Freeze-thawing of cells in the medium contained MC, but without DMSO resulted in a significant reduction of survived cells. The cells, which were pre-cultured in the medium with 0.5% fetal serum (FS) and 0.1% MC, showed higher survival post freeze-thawing in serum-free medium comparing to the cells, which we pre-cultured in the medium with 10% FS. Probl Cryobiol Cryomed 2013; 23(2):116–123.