Защитный эффект метилцеллюлозы при криоконсервировании перевиваемых клеток в бессывороточной среде

• Date: 2013
• Main Authors: Koschiy, Svetlana V., Goltsev, Anatoliy N., Vysekantsev, Igor P., Martsenyuk, Valentina F., Gurina, Tatyana M., Sokol, Larisa V.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
• Subjects: клітини; які перевиваються; кріоконсервування; захисне середовище; метилцелюлоза
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/28
• Journal Title: Problems of Cryobiology and Cryomedicine

There was experimentally shown a possibility to replace blood serum for methylcellulose (MC) in the media for cryopreservation of inoculated cells. Combination of 0.1% MC with 5% dimethyl sufoxide (DMSO) in the preservation media provided the highest indices of post-thaw cell survival. Freeze-thawi...