Влияние диметилсульфоксида и диэтилсульфоксида на термическую денатурацию человеческого сывороточного альбумина

Влияние диметилсульфоксида и диэтилсульфоксида на термическую денатурацию человеческого сывороточного альбумина

The effect of dimethyl sulfoxide (DMSO) and diethyl sulfoxide (DESO) on thermal (heat and cold) denaturation of human serum albumin within temperature range from –20 to90°Cwas investigated by electron and fluorescent spectroscopy. It has been established that at heat induced denaturation in the p...

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Bibliographic Details
Date:2009
Main Authors: Grigoryan, K. R., Markarian, S. A., Aznauryan, M. G.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2009
Subjects:
альбумін
термічна денатурація
електронна спектроскопія
флуоресцентна спектроскопія
диметилсульфоксид
діетилсульфоксид
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/280
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:The effect of dimethyl sulfoxide (DMSO) and diethyl sulfoxide (DESO) on thermal (heat and cold) denaturation of human serum albumin within temperature range from –20 to90°Cwas investigated by electron and fluorescent spectroscopy. It has been established that at heat induced denaturation in the presence of DESO an acid-neutral transition was observed at 47.61°C. It was shown that at cold induced denaturation the direct hydrophobic interactions of sulphoxide molecules with protein surface result in the formation of stable “ice” layer, providing the protein protection from irreversible structure changes.

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