Исследование методом ЭПР влияния замораживания на структурно-функциональное состояние мембран митохондрий

Structure and electron transport function of rat liver mitochondria membranes have been studied by EPR method of spin probes. It is established that at single rapid freezing of organelles down to –196°C there is an increase in the looseness of membranes of mitochondria without its structural chan...

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Bibliographische Detailangaben
Datum:2009
1. Verfasser: Nardid, O. A.
Format: Artikel
Sprache:English
Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2009
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Online Zugang:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/284
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Назва журналу:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Zusammenfassung:Structure and electron transport function of rat liver mitochondria membranes have been studied by EPR method of spin probes. It is established that at single rapid freezing of organelles down to –196°C there is an increase in the looseness of membranes of mitochondria without its structural changes, affecting the condition of proteins of electron transfer chain. Two-fold rapid freezing of suspension of mitochondria results in significant damage of membrane structural organization, inducing the disappearing of thermotropic structural transition in the range of 10°C.