Исследование методом ЭПР влияния замораживания на структурно-функциональное состояние мембран митохондрий

Исследование методом ЭПР влияния замораживания на структурно-функциональное состояние мембран митохондрий

Structure and electron transport function of rat liver mitochondria membranes have been studied by EPR method of spin probes. It is established that at single rapid freezing of organelles down to –196°C there is an increase in the looseness of membranes of mitochondria without its structural chan...

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Bibliographic Details
Date:2009
Main Author: Nardid, O. A.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2009
Subjects:
заморожування-відігрів
мітохондрії
електронний парамагнітний резонанс
відновлення спінового зонда
структурні перебудови
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/284
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:Structure and electron transport function of rat liver mitochondria membranes have been studied by EPR method of spin probes. It is established that at single rapid freezing of organelles down to –196°C there is an increase in the looseness of membranes of mitochondria without its structural changes, affecting the condition of proteins of electron transfer chain. Two-fold rapid freezing of suspension of mitochondria results in significant damage of membrane structural organization, inducing the disappearing of thermotropic structural transition in the range of 10°C.

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