Влияние этапов криоконсервирования методом витрификации в этиленгликоль-сахарозной среде на электрическую проводимость 2-клеточных эмбрионов мышей

• Дата: 2013
• Автори: Smolyaninova, Evgeniya I., Shigimaga, Viktor A., Strikha, Oksana A., Popivnenko, Lyudmila I., Lisina, Ekaterina G.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
• Теми: ембріон миші; електропорація; електричний пробій; вітрифікація; етиленгліколь; кріоконсервування
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/299
• Назва журналу: Problems of Cryobiology and Cryomedicine

In this study the values of specific electric conductivity of 2-cell murine embryos after their exposure in ethylene glycol and sucrose medium and complete cryopreservation cycle by vitrification were determined using the method of impulse conductometry. It has been shown that embryo exposure in vitrifying medium led to an increase in their electric conductivity values in comparison with the control ones ((2.24 ± 0.64) - (3.86 ± 0.68)x10–3 S/m and (1.54 ± 0.110 - 96.12 ± 0.34)x10–3 S/m; respectively). Extension of exposure time from 1.5 min to 3 min led to a decrease in embryo plasma membrane resistance to pulse electric field action. The investigation showed that electric conductivity could be a sensitive diagnostic cell parameter reflecting disturbances in cell morphofunctional integrity at various stages of cryopreservation. Probl Cryobiol Cryomed 2013; 23(3):228–239.