Hydrate structures in partially dehydrated bone marrow cells as studied by 1H NMR spectroscopy

• Datum: 2008
• Hauptverfasser: Turov, V. V., Kerus, S. V., Gun'ko, V. M.
• Format: Artikel
• Sprache: Russisch
• Veröffentlicht: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2008
• Online Zugang: https://surfacezbir.com.ua/index.php/surface/article/view/303
• Назва журналу: Surface

Nonfreezable water and water/organic solvent mixtures bound in partially dehydrated marrow cells released from tubular bones of broiler chickens has been studied using 1H NMR spectroscopy at 210 – 290 K. The 1H NMR spectra of intracellular water include two signals which can be assigned to strongly (SAW, chemical shift of the proton resonance dH = 4…5 ppm) and weakly (WAW, dH = 1,2…1,7 ppm) associated water which can be also divided into weakly (frozen at 260 < T < 273 K) and strongly (unfrozen at T < 260 K) bound intracellular waters. Such solvents as acetonitrile-d3, chloroform-d and DMSO-d6 added to cells differently affect the structure, the Gibbs free energy, and the molecular mobility of intracellular water. A maximum fraction of WAW and a minimum fraction of strongly bound water are observed in intracellular water on addition of acetonitrile to cells. The opposite results are on addition of DMSO which enhances binding, structurization, and the freezing point depression of intracellular water.