Визначення експресії антигенів HLA нейроклітинами людини різного терміну гестації при культивуванні in vitro

• Дата: 2008
• Автори: Lisyanyy, M. I., Lyubich, L. D., Semenova, V. M., Stayno, L. P., Vysotskiy, M. S., Potapova, A. I.
• Формат: Стаття
• Мова: Ukrainian
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2008
• Теми: neurocells; culturing; antigens; нейроклітини; культивування; антигени; нейроклетки; культивирование; антигены
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/358
• Назва журналу: Problems of Cryobiology and Cryomedicine

The HLA expression by human neurocells (NC) of different gestation term and differentiation on the protein and mRNA levels using methods of immunophenotyping and RT-PCR has been studied. Under culturing human NC of 5–6 week’ gestation term in DMEM + retinol acetate the amount and percentage of HLA I and II expressing cells on the protein and mRNA levels were decreased whereas the presence in the culturing medium of the growth factors contributed to preservation (FGF) or increasing (EGF) of positive expressing cells amount. Under culturing in DMEM the HLA I and II expressing Bulbus olfactorius cells number decreased from day 10th to 20th.