ВлиÑние микроинъекций криопротекторов на выживаемоÑÑ‚ÑŒ Ñмбрионов вьюна Misgurnus fossilis L., 1758
Investigated survival rate of loach embryos at a stage late epibole after a microinjection of DMSO, PEG-100, PEG-1500 and formamide (FA) cryoprotectants in area of perivitelline spaces (PS) and yolk sac (YS). It is established that at introduction of DMSO, PEG-100 and PEG-1500 in concentration of 10...
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| Date: | 2008 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | Russian |
| Published: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2008
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| Subjects: | |
| Online Access: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/365 |
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| Journal Title: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and Cryomedicine| Summary: | Investigated survival rate of loach embryos at a stage late epibole after a microinjection of DMSO, PEG-100, PEG-1500 and formamide (FA) cryoprotectants in area of perivitelline spaces (PS) and yolk sac (YS). It is established that at introduction of DMSO, PEG-100 and PEG-1500 in concentration of 10, 50 and 95 % in area of PS and at the subsequent 30-minute incubation in 0,88 and 0,29 M to sucrose, survival rate level decreased: 58,4–48,5, 74,4–53,9 and 79,63–59,6 % accordingly. At FA survival rate level under the same conditions decreased from 7 to 3,6 %. At introduction of PEG-100 and PEG-1500 in concentration 10, 50 and 95 % in YS area at 30-minute incubation in 0,88 and 0,29 M to sucrose level of survival rate had no statistically significant differences and was higher, than at introduction of DMSO and FA. |
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