Цитотоксична активність магнітокерованих нанокомпозитів на основі доксорубіцину на прикладі клітин Saccharomyces Cerevisiae

• Datum: 2016
• Hauptverfasser: Turanska, S. P., Kusyak, A. P., Petranovska, A. L., Gorobez', S. V., Turov, V. V., Gorbyk, P. P.
• Format: Artikel
• Sprache: Ukrainisch
• Veröffentlicht: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2016
• Schlagworte: нанорозмірний однодоменний магнетит; нанокомпозити Fe3O4/доксорубіцин; Fe3O4/SiO2/доксорубіцин; Fe3O4/TiO2/доксорубіцин; Fe3O4/гідроксіапатит/доксорубіцин; доксорубіцин; дріжджові клітини; цитотоксичність; проліферація
• Online Zugang: https://www.cpts.com.ua/index.php/cpts/article/view/383
• Назва журналу: Chemistry, Physics and Technology of Surface

The aim of the work is investigation of cytotoxic influence of the magnetocarried polyfunctional nanocomposites based on single-domain magnetite (Fe3O4) and anthracycline antibiotic doxorubicin (DOX) on yeast cells Saccharomyces cerevisiae. Magnetite was synthesized according to the Elmor reaction. For investigations we used a fraction of particles with sizes of 6-23 nm. Specific surface area of the samples studied was S = 105–180 m2/g. Magnetosensitive nanocomposites Fe3O4/DOX, Fe3O4/SiO2/DOX, Fe3O4/TiO2/DOX, Fe3O4/ hydroxyapatite (HA)/DOX were synthesized. To obtain Fe3O4/SiO2 nanocomposites, we used tetraethoxysilane (TEOS) as a modifying agent. The technique for synthesis of nanocomposites consisting of magnetite modified with silica dioxide involves hydrolysis of TEOS. The coating was 0.2 g of SiO2 on 1 g of magnetite. Specific surface area of the nanocomposite increases from 105 m2/g (for unmodified magnetite) to 130 m2/g. To obtain Fe3O4/TiO2 nanocomposites, we used n-butylorthotitanate as a modifying agent. The technique based on the reaction of conversion of n-butylorthotitanate on the magnetite surface into ТіО2. This conversion consists in hydrolysis of n-butylorthotitanate followed by condensation of hydrolysis products with formation of amorphous ТіО 2. Modification of the surface of nanosized magnetite with hydroxyapatite (obtaining of Fe3O4/HА composites) was carried out by the reaction: 10 Са(NO3)2 + 6 (NH4)2HPO4 + 8 NH4 ОН ? Са10(PO4)6( ОН)2 + 20 NH4NO3. We realized immobilization of DOX on the surfaces of magnetite Fe3O4 and nanostructures Fe3O4/SiO2, Fe3O4/TiO2, Fe3O4/HA by adsorption method from saline environment. Bioactivity of the nanocomposites was determined in the study of viability of cells by a cytochemical method using optical microscopy and Goryaev chamber with registration of concentration change for cells in suspensions containing nanocomposites, yeast cells, minimal synthetic nutrient medium and saline. Fe3O4/DOX, Fe3O4/SiO2/DOX, Fe3O4/TiO2/DOX, Fe3O4/HA/DOX nanocomposites had cytotoxic and antiproliferative activity with respect to Saccharomyces cerevisiae cells, which were characteristic for a free form of doxorubicin. Using the chosen objects, we worked out rather effective, reliable, safe and relatively inexpensive technique for control of cytotoxic activity of nanocomposites, which could be actual for use in development of new medical magnetocarried remedies of targeted delivery, in particular, for oncology.