Моделювання кісткової тканини нанокомпозитними системами на основі гідроксиапатиту − альбуміну − желатини та їхні властивості

• Datum: 2010
• Hauptverfasser: Golovan, A. P., Rugal, A. A., Gun`'ko, V. M., Barvinchenko, V. N., Skubiszewska-Zięba, Ya., Leboda, R., Krupska, T. V., Turov, V. V.
• Format: Artikel
• Sprache: Russisch
• Veröffentlicht: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2010
• Online Zugang: https://surfacezbir.com.ua/index.php/surface/article/view/417
• Назва журналу: Surface

Nanocomposite material on the basis of nano-hydroxyapatite (HAP) and proteins, gelatine and serum albumin (HGA) was synthesized. Its hydration properties were found to be similar to those of a natural bone tissue. Adsorption interactions between the protein macromolecules and hydroxyapatite particles were confirmed by FTIR-spectroscopy. It was found that the mineral component of the HGA composite, as well as HAP, contains significant amount of carbonate groups. According to DSC, the radius of water aggregates of weakly bound water in  the hydrated sample of HGA is in the range of 1.5 − 80 nm. The photon-correlation spectroscopy has indicated that the particle size of the synthesized materials after their ultrasonic treatment is in the range of 80 − 100 nm. It is shown that the water adsorbed by particles of HGA nanocomposite, when contacts with organic substances displays heterogeneous, cluster structure. Depending on the composition of the organic medium, water can exist in strongly associated or weakly associated state, and partially passes into a liquid phase if the latter contains electrono-donor solvents, acetonitrile or DMSO. The properties of the synthesized HGA nanocomposite in aqueous-organic solutions are close to similar characteristics of a natural bone material. HGA showed good biocompatibility with cellular objects (yeast cells). Synthesized material significantly accelerates the processes of cell live activity what makes it a promising model for testing as a synthetic substitute for bone tissue.