Іммобілізація уреази на полі(3-меркаптопропіл)силоксанових носіях
Sol-gel method was used to synthesize poly(3-mercaptopropyl)siloxane xerogels due to co-hydrolysis of tetraethoxysilane and 3-mercaptopropyltriethoxysilane. When the ratio of initial reagents was 1:1, nonporous matrixes with high content of functional groups in the surface layer were formed. Composi...
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| Datum: | 2011 |
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| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | Ukrainisch |
| Veröffentlicht: |
Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine
2011
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| Online Zugang: | https://surfacezbir.com.ua/index.php/surface/article/view/439 |
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| Назва журналу: | Surface |
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Surface| Zusammenfassung: | Sol-gel method was used to synthesize poly(3-mercaptopropyl)siloxane xerogels due to co-hydrolysis of tetraethoxysilane and 3-mercaptopropyltriethoxysilane. When the ratio of initial reagents was 1:1, nonporous matrixes with high content of functional groups in the surface layer were formed. Composition, structure, and structural-adsorption characteristics of the synthesized materials were studied. Silicas functionalized with 3-mercaptopropyl groups were used to study urease immobilization by adsorption, covalent binding, and incorporation in polysiloxane network during synthesis. The highest levels of immobilization (91 %) and activity retention (55 %) were observed when urease was incorporated into network during synthesis. Urease immobilized by this method retains its activity at 30% for 170 days. |
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