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Іммобілізація уреази на полі(3-меркаптопропіл)силоксанових носіях

Sol-gel method was used to synthesize poly(3-mercaptopropyl)siloxane xerogels due to co-hydrolysis of tetraethoxysilane and 3-mercaptopropyltriethoxysilane. When the ratio of initial reagents was 1:1, nonporous matrixes with high content of functional groups in the surface layer were formed. Composi...

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Date:2011
Author Affiliations:
  • R. P. Pоgoriliy — Інститут хімії поверхні ім. О.О. Чуйка Національної академії наук України
  • V. P. Goncharik — Інститут хімії поверхні ім. О.О. Чуйка Національної академії наук України
  • Yu. L. Zub — Інститут хімії поверхні ім. О.О. Чуйка Національної академії наук України
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Main Authors: Pоgoriliy, R. P., Goncharik, V. P., Zub, Yu. L.
Format: Article
Language:Ukrainian
Published: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2011
Online Access:https://surfacezbir.com.ua/index.php/surface/article/view/439
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Journal Title:Surface
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Summary:Sol-gel method was used to synthesize poly(3-mercaptopropyl)siloxane xerogels due to co-hydrolysis of tetraethoxysilane and 3-mercaptopropyltriethoxysilane. When the ratio of initial reagents was 1:1, nonporous matrixes with high content of functional groups in the surface layer were formed. Composition, structure, and structural-adsorption characteristics of the synthesized materials were studied. Silicas functionalized with 3-mercaptopropyl groups were used to study urease immobilization by adsorption, covalent binding, and incorporation in polysiloxane network during synthesis. The highest levels of immobilization (91 %) and activity retention (55 %) were observed when urease was incorporated into network during synthesis. Urease immobilized by this method retains its activity at 30% for 170 days.

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