Анализ влияния различных этапов криоконсервирования методом витрификации в этиленгликоль-сахарозной среде на жизнеспособность 2-клеточных эмбрионов мыши

The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure...

Повний опис

Збережено в:
Бібліографічні деталі
Дата:2007
Автори: Smolyaninova, Ye. I., Pishko, O. V., Lisina, E. G., Kolesnikova, A. A., Rozanov, L. F.
Формат: Стаття
Мова:English
Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
Теми:
Онлайн доступ:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/485
Теги: Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
Назва журналу:Problems of Cryobiology and Cryomedicine

Репозитарії

Problems of Cryobiology and Cryomedicine
Опис
Резюме:The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure in freezing medium and freeze-thawing are mostly responsible for embryo cryoinjury. Basing on the literature reports and own data the role of osmotic and mechanical factors in cell injury at the rapid freezing stages is discussed.