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The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure...
Збережено в:
Дата: | 2007 |
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Автори: | , , , , |
Формат: | Стаття |
Мова: | English |
Опубліковано: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2007
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Теми: | |
Онлайн доступ: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/485 |
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Назва журналу: | Problems of Cryobiology and Cryomedicine |
Репозитарії
Problems of Cryobiology and CryomedicineРезюме: | The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure in freezing medium and freeze-thawing are mostly responsible for embryo cryoinjury. Basing on the literature reports and own data the role of osmotic and mechanical factors in cell injury at the rapid freezing stages is discussed. |
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