Анализ влияния различных этапов криоконсервирования методом витрификации в этиленгликоль-сахарозной среде на жизнеспособность 2-клеточных эмбрионов мыши

Анализ влияния различных этапов криоконсервирования методом витрификации в этиленгликоль-сахарозной среде на жизнеспособность 2-клеточных эмбрионов мыши

The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure...

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Bibliographic Details
Date:2007
Main Authors: Smolyaninova, Ye. I., Pishko, O. V., Lisina, E. G., Kolesnikova, A. A., Rozanov, L. F.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
Subjects:
вітрифікація
2-клітинний ембріон миші
етиленгліколь
сахароза
кріоконсервування
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/485
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure in freezing medium and freeze-thawing are mostly responsible for embryo cryoinjury. Basing on the literature reports and own data the role of osmotic and mechanical factors in cell injury at the rapid freezing stages is discussed.

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