ПЭО-1500 ингибирует Са2+-АТРазу эритроцитов человека

• Дата: 2007
• Автори: Zemlyanskikh, N. G., Nikolchenko, A. Yu., Babijchuk, L. A.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
• Теми: erythrocytes; Ca2 -ATPase; cryopreservation; cryoprotectant; polyethylene oxide; эритроциты; Cа2 -АТРаза; криоконсервирование; криопротектор; полиэтиленоксид; еритроцити; кріоконсервування; кріопротектор; поліетиленоксид
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/511
• Назва журналу: Problems of Cryobiology and Cryomedicine

Human erythrocyte Ca2+-ATPase provides the maintenance and regulation of intercellular calcium level. Change in its functional activity under cryopreservation plays an important role in cell survival and structural integrity preservation. PEO-1500 exocellular cryoprotectant inhibits erythrocyte Ca2+-ATPase activity at incubation stage but the following freeze-thawing cycle (–196...37°C) in its presence does not affect the settled regimen of this enzyme functioning. The inhibition caused by PEO-1500 effect has a reversible character and the erythrocyte Ca2+-ATPase activity is recovered after its dilution in cell suspension that is essential for cryopreserved cell normal functioning in blood bed after transfusion. The inhibition of Ca2+-ATPase activity is apparently associated to physical and chemical membrane modification under this compound effect.