Исследование влияния криопротекторов на липосомы из суммарных липидов сперматозоидов собаки

• Дата: 2007
• Автори: Linnik, T. P., Dyubko, T. S., Yegorov, M. I., Pivovarenko, V. G.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
• Теми: собака; сперматозоїди; ліпіди; кріопротектори; флуоресцентний зонд; ФМЕ; сперматозоиды; липиды; криопротекторы; флуоресцентный зонд; dog; spermatozoa; lipids; cryoprotectants; fluorescent probe; FME
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/513
• Назва журналу: Problems of Cryobiology and Cryomedicine

The mechanism of interaction between the cryoprotective agents (CPAs): ethylene glycol (EG), glycerol and N,N-dimethylformamide (DMFA) with lipid bilayer of liposomes formed from total lipids of canine spermatozoa by fluorescent spectroscopy using new fluorescent probe of flavonoid origin: 3-hydroxy-4’-(N,N-dimethylamine)flavon (FME). It has been established that among the studied CPAs (glycerol, EG, DMFA) the highest effect on the form of FME fluorescence spectrum associated to lipid bilayer is rendered by DMFA. The effect rate of the studies CPAs on liposomes is determined by hydrophobic-hydrophilic balance of their molecules. DMFA predominantly affects lipid bilayer by hydrophobic interaction, and glycerol and EG mainly influence hydrate layer of polar “heads” of phospholipids by forming intermolecular hydrogen bonds.