Изучение влиÑÐ½Ð¸Ñ Ð½Ð¸Ð·ÐºÐ¸Ñ… температур на ÑохранноÑÑ‚ÑŒ инфекционноÑти, антигенной Ñтруктуры и ДÐК вируÑа инфекционного ринотрахеита
Effect of freezing and long-term storage at moderately low temperatures and in liquid nitrogen on the integrity of infectious properties, antigen structure and DNA of vaccine and industrial strains of infectious bovine rhinotracheitis (IBRT) was studied. It has been established that infectivity in t...
Збережено в:
Дата: | 2007 |
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Автори: | , , |
Формат: | Стаття |
Мова: | English |
Опубліковано: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2007
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Теми: | |
Онлайн доступ: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/516 |
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Назва журналу: | Problems of Cryobiology and Cryomedicine |
Репозитарії
Problems of Cryobiology and CryomedicineРезюме: | Effect of freezing and long-term storage at moderately low temperatures and in liquid nitrogen on the integrity of infectious properties, antigen structure and DNA of vaccine and industrial strains of infectious bovine rhinotracheitis (IBRT) was studied. It has been established that infectivity in the first passage was expressed by the viruses stored for 8 months and 18 months under moderately low temperatures as well as for 30 months in liquid nitrogen. Immune enzyme analysis has shown the absence of glycoprotein B in thawed virus samples, stored within the range from –18 to –30°C. Specificity of amplified DNA fragment is confirmed by PCR for all studied samples of IBRT virus. |
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