Нові флуоресцентні барвники як зонди для дослідження сперматозоїдів собак у кріозахисних середовищах

Нові флуоресцентні барвники як зонди для дослідження сперматозоїдів собак у кріозахисних середовищах

Possibilities of using DSM, E-176, 3-DAB, FME fluorescent dyes for examining the effect of cryoprotective agents on canine spermatozoa were studied with the methods of fluorescent spectroscopy and microscopy. FME and 3-DAB dyes were found to be capable of solving the tasks set and DSM, E-176 ones ha...

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Bibliographic Details
Date:2006
Main Authors: Еgorov, M. I., Dyubko, T. S., Linnik, T. P., Belonozhko, A. P., Еrmolenko, I. G., Semenova, O. N., Patsenker, L. D., Pivovarenko, V. G., Povrozin, E. A.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2006
Subjects:
сперматозоїди собак
диметилформамід
етиленгліколь
флуоресцентні зонди
ДСМ
Є-176
3-ДАБ
ФМЄ
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/612
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:Possibilities of using DSM, E-176, 3-DAB, FME fluorescent dyes for examining the effect of cryoprotective agents on canine spermatozoa were studied with the methods of fluorescent spectroscopy and microscopy. FME and 3-DAB dyes were found to be capable of solving the tasks set and DSM, E-176 ones had a limitation due to quite a strong fluorescence of cryoprotectants’ solutions. Studied fluorescent dyes were demonstrated as differently affecting cell motility.

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