Роль переохлаждения в сохранении клоногенных свойств фибробластоподобных клеток-предшественников эмбриональной печени человека после криоконсервирования

Роль переохлаждения в сохранении клоногенных свойств фибробластоподобных клеток-предшественников эмбриональной печени человека после криоконсервирования

Effect of different freezing conditions on human fetal liver (HFL) cell viability and colony-forming activity of HFL fibroblast-like progenitor cells (FPCs) was investigated. This progenitor type was established to be a high sensitive to a damaging effect of overcooling. Overcooling reduction at a s...

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Bibliographic Details
Date:2006
Main Authors: Skorobogatova, N. G., Grischuk, V. P., Petrenko, A. Yu.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2006
Subjects:
кріоконсервування
фібробластоподібні клітини-попередники
ембріональна печінка людини
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/613
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:Effect of different freezing conditions on human fetal liver (HFL) cell viability and colony-forming activity of HFL fibroblast-like progenitor cells (FPCs) was investigated. This progenitor type was established to be a high sensitive to a damaging effect of overcooling. Overcooling reduction at a slow three-step freezing enables a successful cryopreservation of FPCs in primary suspension of HFL cells with preservation of their clonogenic properties.

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