Изучение влиÑÐ½Ð¸Ñ Ñ…Ð¾Ð»Ð¾Ð´Ð¾Ð²Ð¾Ð³Ð¾ Ð·Ð°ÐºÐ°Ð»Ð¸Ð²Ð°Ð½Ð¸Ñ ÐºÐ°Ñ€Ñ‚Ð¾Ñ„ÐµÐ»Ñ Ð½Ð° ÑохранноÑть мериÑтем, криоконÑервированных медленным замораживанием
Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in com...
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Datum: | 2006 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | English |
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Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2006
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Online Zugang: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/617 |
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Назва журналу: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and CryomedicineZusammenfassung: | Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in comparison with the same index in non-hardened meristems. Potato apex hardening is inexpedient when using 1,2-PD. Observation for cryopreserved meristem development within 30 days has demonstrated that treatment with cryoprotectants and freezing inhibit an apex growth in comparison with the control. 1,2-PD occurred to be less toxic. DMSO treatment can cause callus-genesis. |
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