Влияние глицерина, этиленгликоля на морфофункциональные характеристики сперматозоидов собак на разных этапах криоконсервирования

• Дата: 2004
• Автори: Egorov, M. I., Kopeika, E. F., Linnik, T. P., Kuchkov, I. N.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2004
• Теми: spermatozoa; motility; equilibration; cryopreservation; glycerol; ethylene glycol; сперматозоїди; рухливість; еквілібрація; кріоконсервування; гліцерин; етиленгліколь; сперматозоиды; подвижность; эквилибрация; криоконсервирование; глицерин; этиленгликоль
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/655
• Назва журналу: Problems of Cryobiology and Cryomedicine

There has been studied the effect of glycerol and ethylene glycol (EG) on survival of dogs’ spermatozoa (DS) after freeze-thawing depending on the concentration of cryoprotectants, cooling regimens and equilibration time, as well as the change in DS cell volume after the cryoprotectants being introduced into suspension by means of turbidimetric method. The highest motility of DS was revealed after cryopreservation under EG protection with final concentration of 7% when being frozen at the first stage with the rate of 3°C/min with no preliminary equilibration. It has been shown that 7% EG causes quite less alterations in cell volume if compared to glycerol.