Дослідження впливу поліетиленгліколів великої молекулярної маси і декількох полоксамерів на цілісність і плинність мембран еритроцитів методом спінових зондів

• Дата: 2019
• Автори: Ivanov, L. V., Scherbak, O. V., Kartel, M. T.
• Формат: Стаття
• Мова: Українська
• Опубліковано: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2019
• Теми: плинність ліпідів; плавлення; мембрани еритроцитів; аксон сідничного нерва; поліетиленгліколі; метод спінових зондів; дегідратація клітин; цілісність еритроцитів
• Онлайн доступ: https://surfacezbir.com.ua/index.php/surface/article/view/671
• Назва журналу: Surface

Spin-probe methods studied the mechanisms of influence of polyethyleneglycols (PEG) on the fluidity of lipid membranes of erythrocytes, the mechanisms of possible membrane lipid membrane and mechanisms of violation of the integrity of membranes of red blood cells over time. The administration of 15% PEG-1500 in human erythrocytes and rats showed increased fluidity of membranes of erythrocytes by 16 or 30 %, but then membrane fluidity decreased due to dehydration and cell compression. The introduction of hydrophilic poloxamer 407 and 338 into erythrocytes led to the melting of lipid membranes (the effect of increasing the fluidity of membranes 50-70 %), but the integrity of most cells remained. Before the destruction of the membranes, the viscosity of the cytosol decreased sharply and anisotropy of the EPR spectra was observed, which was associated with significant changes in the structure of the red cell cytosol. Involving the experiment, poloxamers containing ethylene glycol and propylene glycol, showed the mechanisms of genuine melting of membranes with a maximum increase in lipidity (70 % effect), which is bounded by partial hemolysis and cell coagulation. Melting of phospholipids of membranes of erythrocytes under the action of poloxamers 407 and 338 showed that the real reason for melting are hydrophobic methylene groups of PEG or propylene glycol poloxamers, which destroy the structure of cell membranes from all directions.