Adsorption of bioactive molecules of medicinal plants
The adsorption of some herbal bioactive polyphenolics (anthraquinones, flavons) from solutions of different polarity onto a surface of highly dispersed silica (entherosorbent Silics) is studied. It is found that the structure of adsorption complexes and the mechanism of their formation depend essent...
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| Date: | 2001 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine
2001
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| Online Access: | https://surfacezbir.com.ua/index.php/surface/article/view/69 |
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Surface| _version_ | 1869291156436680704 |
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| author | Pogorelyi, V. K. Barvinchenko, V. N. Lipkovska, N. A. Turov, V. V. Chuiko, A. A. |
| author_facet | Pogorelyi, V. K. Barvinchenko, V. N. Lipkovska, N. A. Turov, V. V. Chuiko, A. A. |
| author_institution_txt_mv | [
{
"author": "V. K. Pogorelyi",
"institution": "Інститут хімії поверхні НАН України"
},
{
"author": "V. N. Barvinchenko",
"institution": "Інститут хімії поверхні НАН України"
},
{
"author": "N. A. Lipkovska",
"institution": "Інститут хімії поверхні НАН України"
},
{
"author": "V. V. Turov",
"institution": "Інститут хімії поверхні НАН України"
},
{
"author": "A. A. Chuiko",
"institution": "Інститут хімії поверхні НАН України"
}
] |
| author_sort | Pogorelyi, V. K. |
| baseUrl_str | |
| collection | OJS |
| datestamp_date | 2018-11-27T09:42:39Z |
| description | The adsorption of some herbal bioactive polyphenolics (anthraquinones, flavons) from solutions of different polarity onto a surface of highly dispersed silica (entherosorbent Silics) is studied. It is found that the structure of adsorption complexes and the mechanism of their formation depend essentially on the solvent nature. Preliminary clinical researches have shown high medecinal activity of Phytosil composites regarding children’s herpes stomatitis. The effectiveness of Phytosil composite corresponds to the level of thymic immunostimulators, but its application makes the process of treatment simpler and cheaper. |
| first_indexed | 2025-07-22T19:30:03Z |
| format | Article |
| fulltext |
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ADsoRPTIoг{ oF BIoACTtvЕ гиOLЕСULЕS
oF IиЕDIСIГ\IAL РLAl.iTS
V.K.Pogorelуi' V.N. Barvinсhеnko, N.A. Lipkovska, V.V. Turov,
and A.A. Chuiko
Institutе of Suфcе Сhemistry' Nаtioпаl Аcаdеmу of Sсieпсes
Geп. Nаurnot, str. ]7, Kуiу-]61, 03680 L\KRАINЕ
Abstraсt
The adsoгption of some hегbal bioaсtivе pоlyphеnoliсs (anthraquinonеs, flavоns)
fгom solutions of diffеrеnt polaritу onto a suгfaсе of Ыghlу dispегsеd siliсa (enthеrosorbеnt
Siliсs) is studiеd It is fоund that thе Struсturе of adsoгption сomplеxes and thе mеоhanism
of thеiг formation dеpend еssentially on thе solvеnt natuге. Pгеliminary сliniсal геseaгсhеs
havе shown high mеdесinal aсtivitу of Phytosil сomposites rеgarding сhildгеn's hегpеs
stomatitis. Thе еffесtivеnеss oГ Phуtоsil соmpositе соггеsponds to thе lеvеl of thymiс
immunostimulatoгs, but its appliсation makеs thе pгoсеss of tгеatmеnt simplеr and сhеapег,
In thе last fеw yеaгs sсiеntifiс workегs of thе Institute of SuгГaсе Сhemistry of thе
NAS of Ukrainе сonduсtеd сompгehеnsivе phуsiсoсhеmiсal and mеdiсo.biolоgiсal studiеs
dirесtеd аt dеvеloping a nец, genегation of сompositе mеdiсinal pгеpaгations of thе
Phytosil sегiеs inсoгpoгating natil'е сompounds and еnthегosoгbеnt. Thе thегapеutiс
aсtivity of biomolесulеs and thеiг сomplехеs in similar сompоsitеs is сombined with a
bгoad gamut of dеtoxiсating aсtion of entегosorbent Siliсs bаsеd on a highlу dispеrsеd
siliсa Il]'
It is natuгal to assume that thегapеutic pгopeгtiеs of mediсinal plants aге
dеtегminеd not by a single substanсe (еven if it is thе most aсtive оnе) but by thе wholе
сornplех of сuгativе substanсеs and its natuгal еnviгonmеnt For this геason, whеn foгming
a phytоsoгptivе compositе onе should not usе, as faг as possiblе, traditional galеniсals. It is
much mоrе еxpеdiеnt to usе mеdiсinal hеrbs in thе highlу dispеrsеd foгm' Whеn applуing
this approaсh' thе bioaсtivе сomplех of a mеdiсinal plant that hаs not bееn subjесtеd tо
damaging еffесt of tеmpегatuге and ехtгaсtant or solvеnt pгеsеrves its natuгal сomposition,
molесular weight and ratio of сomрonеnts, оonformation and environmеnt, and, as a
сonsrquеnсе' it wiu ехhibit its maхimum thеraреutiс aсtivity, Bеsidеs, thе phaгmaсologiсal
aсtion of natuгal сompounds in сompositеs of this typе will bе aссompaniеd by thе
detoxiсating еffeсt of an еntегosoгbеnt' In this way it is possiblе to rulе out сomplеtеly oг
to dесrеasе сonsidегablу thе pгobabilitу of bloсking thе сurativе substanсеs of a
phytoсomplех by endо- oг ехotoхins, wЫоh rеsults in a substantial inсrеasе in thе
bioavailability and еffrсienсy of thеsе substanсеs.
Тhе abovе-mеntionеd givеs ground to beliеvе that thе dеvеlopеd mеdiсamеnts of
thе Phytosil sеriеs, in сomparison with thе familiar foгms (екtгaсts, tinсturеs, еtс.), will
offег a numbеr of advantagеs: a) a moге Ьгoad gamut of сurativе pгopertiеs, b) еnhanсеd
intеnsity of aсtion, с) prolongation and diгeсtionality, d) сompaгablе theгapеutiс еffесt at a
lowеr dosе, е) shoгtеning of tегms of mеdiсal trеatmеnt, Г; есоnomiсal and tесhnologiсal
еffесtivеness of produсtion, g) simplе mеthods of appliсatiоn
Thus, with thе viеw of сгеating a nеW gеnеration of сompositе mеdiсinаl
pгеparations the puгposeful геsеarсlrеs wеге сonduсtеd in thе Гollowing thгее diгесtions:
)6O nnrvv YI
- 199'7.-
етosoгbеnt,
р
l Agеnt fог
:е's Dеgrее
rse Siliсas:
i0r
1. asсeгtainmеnt of гegulaг pattегns of soгptivе intегfaсial intеraсtions bеtwееn moleсцlеs
of natuгal bioaсtivе сompounds and еntегosoгbеnt in vaгious mеdia inсluding
physiologiс onеs;
2. aesignlng of qualitative and quantitativе сompositions of a сurative сompositе;
o.ujopйg of mеthods foг blеnding its сonstituеnts into a singlе mеdicamеntous form;
working out of proсessing tесhnologу for suсh a foгm;
з сaгrуin! out of iliniсal trйls and, on the basis of the outсome of thе trials, optimization
of tй. йmposition and mеthods of aрpliсation of a сurativе сompositе.
The indispеnsablе сondition whiсh definеs thе possibilitу of сrеаting сurativе
соmpositеs of soгptivе aсtion is rеvеrsibilitу of adsorption of bioaсtive molесules and thеir
.o'pl.*"* on th; suгГaсе of an еntегosorbеnt in a phуsiologiсal salt solution. Hеnсе,
praсiiсal dеvеlopments of mеdiсinal pгеparations requiге knowlеdgе of fundamеntal laws
of adsoгption intегaсtions.
Ii is known [2] that the most aсtivе bioсompounds obtained from mеdiсinal plants
inсludе flavonoids, сardiostеroids' alkaloids as wеll as dеrivatives of anthraquinonе,
сЦmarin, еtс. Wе havе studiеd adsoгption of 9,1O-anthraquiononе (I), 1,2.dihуdгoхy-9,10.
anthraquinonе (alizагin, II), 3,5,7,3,,4'-pеntahydroxyflavonе (querсеtin, III) usеd in thе
".p."i'у
of modЪl molесulеs or bioaсtivе сompounds of
oHolll
thе anthraquinonе and flavonoid sеriеs. Thе adsoгbеnt has bееn pyrogеnic highly dispeгsеd
siliсa with paгtiсlе sizes of d < og mm and spесifiс suгfaсе aгеa of З80 m2 g-'. Thе
adsoгption has bееn еffесtеd fгom various mеdia, namеlу inегt aprotiо solvеnts (hехane,
."йЪi.i' polar protiс solvеnts (rr,atеr, alсohol, and thеiг mixturеs).
-
Rеaсtivе sitеs of sorbatе molесulеs inсludе isolatеd сaгbonyl gгoups (еleсtron
donors),speсiеswithanintramolесularhуdгogеnbond(oH...o=C),andhydroхyls.in.o-
and и-positions сapable of aсting as donoгs йd u...ptoгs of pгotons in intегmoleсular
inteгaсtions. Thеsе fеatuгеs of thJsorbatе сhеmiсal struсturе imply variеty of mесhanisms
of spесifiс Sorрtlon оn hуdroxylated suгfaсе of Sioz mainly thгough foгmation of hуdrоgеn
bonds with *гoups oH on tйе suгfaсе oг of сooгdination bonds of siliсon atoms with
з02
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:еdiсinal plants
xthгaquinonе,
.:r,droxy-9,10-
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7 .. nесhanisms
3с. :fhydrogen
b-. аtoms with
mono- or bidеntatе ligands [3]. Thе еx1эегirnеntal pгoсеdurеs appliеd havе bееn dеsсiibеd
in Rеt [a]
It has bееn found that сompounds l - IiI in thеiг molесulaг form arе not sorbеd bv
siliсa fгom aquеous' alсoholiс. ог watеr-alсohol sоlutions. Тhis faсt pгo-vidеs еvidеnсе for
сompеtition among ргotiс solvеnt molесulеs аnd adsorbatе mo-leсulеs foг reaсtivе sites on
thе suгfaсе. Bеsidеs, it also gives еvidеnсе for pгеdomi-nanсе of solvation еffесt ovег
adsoгption one' Aлthгaquinonе is not soгbеd еvеn from toluеnе. Thе mоlесulaг adsoгption
is observеd only in thе сase of inегt solvеnt rr-heхanе' Thе linеaг сharaсtег оf the гесоrded
adsorption isothегms that have no paгts of saturation is attгibutеd to thе faсt that thе
highest possible adsoгption valuе сonsiderably eхсееds thе solubility valuе of сompounds
I_III in r-hеxanе [4]
In going from I to II thе adsoгbabilitу substantially inсгеasеs. Consеquеntlу,
hуdroхy gгoups of alizarin aсt as additional геaсtivе sitеs that sееm to paгtakе in the
intегaсtion with thе surГacе thгough formation of hydrogеn bonds of thе o-H...o-H tyре
with hydгoxyls on the suгfaсе or in its hydratеd layег' Thе important гоlе of ц,itег
molесules on thе intегfaсe in thе pгoсеss of adsoгptiоn of pгotophiliс molесulеs (I) oг
protogеniс molесцles (II) is evidеnсеd foг by thе геsults aсhiеvеd bу сomparing maximum
adsoгption valuеs гесoгdеd in thе сasе of the staгting sоrbent and dehydratеd sогbеnt
obtainеd aftег thе hеat tгeatmеnt in air foг 2 h at 190.C. It tuгns out that еvеn a paгtia]
геmoval of wateг fгom a solvation сovег of siliсa maгkеdly deсгеasеs thе adsоrption valuе,
whiсh pгovidеs еvidеnсе for paпiсipation of watег molесulеs of a nеaг-suгfaсе laуеr in
binding of pгotoaсtive adsoгbatе molесulеs,
Тhe сompaгative analysis of thе inГгarеd and еlесtгoniс speсtra гесordеd for
moleсuiеs in solution and on thе siliсa suгfaсе madе it possiblе to form somе judgеments
on thе mесhanism of adsorption ftom an inегt solvеnt. When сompound (I) passеs from thе
solution onto thе suгГaсе' onе сan obsегvе (sее Tablе l) a shift oГthе valеnсе vibгation
band of thе сaгbonуl grоup (Aи = lО сm_'1 into thе low frеquеnсy rеgion, as wеll as a
bгoadеning and bathoоhromiс shift оf thе band of transitiоn rr-+o* (Ь'1" = 1О nm) Тhе
сhangеs in thе infrared and еlесtгoniс spесtra obsеrved duгing adsoгption aге duе to
foгmation of hydгogen bonds betweеn gгoups C=o of сompound (I) and hуdгoхyl gгoups
оfthе suгГaсе or its hydratеd сovеr'
=Si-OH'..O=C oг =Si_oH.'.(o-н),...o:С
I
н
Aлalogous сhangеs aге also dеtесtеd in the еlесtroniс speсtгa of aiizarin. Thе two
bands of transition 7Г-+7т obsегvеd in thе сasе of solutiоn (sее Tablе 1) and rеlatеd to
groups С:o bound with intramoleсulaг hydгogеn bonds oг to frее groups C:o in thе
Spесtrum rесoгdеd foг adsогbеd сompound (II) manifеst thеmsеIves in the fогm of a
diffusе linе with Ьo, = 334 nm. Two bands of transition rr.)7r сhaгaсteгistiс of thе
molесulaг foгm of сompound (II) in thе spесtrum foг thе adsoгbеd statе manifеst
thеmsеlves as a broad linе with 2.o, = 4З.7 nm' Thегеforе, alizaгin is also adsoгbеd on thе
suгГaсе of Sioz thгоugh formation of hydrоgеn bоnds bеtwееn С=o and o_H groups аnd
hуdroхуls of thе suгfaсе, with paпiсipation of intегmеdiatе molесulеs of watеr bеing
possiblе It should be noted that in thе сasе of similaг sуstеms thе paпiсipation of watег
molесulеs bound to thе suгfaсе in thе Гoгmatiоn of adsoгptiоn сomplехеs is еspесially
еffесtivе' Thе point is that groups o_H both on thе surfaсе and in molесulеs of соmpound
(II) in viпuе of thеiг aсidiс сharaсtег рossеss a moге prоfound pгоtogеniс саpaсity, whllе
watег molесules еxhibit idеntiсal pгoton-donating and pгoton-aссеpting сharaсtегistiсs
with геfеrеnсе to hydгogеn bonds.
303
Sinсe adsoгption of сompounds (i) and (II) on thе siiiсa suгflaсе from solutions of
inегt solvеnts is геvегsiblе аnd сausеd bу foгmation oГ hуdгogеn bonds whosе strеngth
doеs not ехсееd intensity of thегmal effеcts of solvation of pгotoaсtivе molесulеs in
physiologiсal media, onе maу assumе that adsoгption intеraсtions in systеms of a similaг
iуpe йllЪot ехегt a nеgativе influenсе on thе еffiсaсy of a сuгativе сompositе.
As it has alrеadу bееn mentionеd abovе, сompounds (I) _ (III) in thеir molесulaг
foгm aге not sorbеd on siliсa from watег oг from wateг-alсohol solutions' Sinсе alizarin
ФKo = 5 47) and quегсеtin ФKo = 1 41) tn polar solvents еxist in two foгms (molесulaг and
jеpгotonatеd) whose reaсtivitiеs with гesреоt to thе suгfaсе sitеs diffеrs substantially, it
was of intегеst for us to invеstigate thе adsoгbability of сompounds (iI) and (IП)
dеpending on pН of wateг.alсohol solution (1:1). As thе pH valuеs foг thе onsеt of
adiorptio-n and dissociation of silanol groups сoinсidе, onе may assumе that dеprotonatеd
silanol groups (=Sio-...H_oAr) aсt in thе сapaсity оf primary adsorption sitеs foг bonding
of polylhеnols' Howеvеr, thе adsoгbabilitiеs of thе polyphеnols studiеd are low
(u^"*=.ц*10-7 and 1х10.7 mol g-lfoг сompounds (II) and (II) геspесtivеly) еvеn in thе
сasе of rеlativеlу сonсentгаtеd solutions (Co = 10_3 mol l_'). Thе maximum adsoгption
valuе foг qu",".iin is сonsidеrably lowег than that foг alizaгin in spitе of thе prеsеnсе of a
laгgе nu.Ъеr of gгoups oH in thе molеculе of thе foгmеr. It is pгobablе that with
inсТeasing numbеrЪf hydгoху gгoups in thе adsoгbatе molесule thе total thегmal еffесt of
solvation in pгotoaсtiv; solvешs (watег' alсohоl) inсгеasеs, whiсh' natuгally, hindеrs thе
passagе of a polyphеnol from solution onto thе surfaсе. Aлd, finaliy, in thе сase of
qu...Ъtin 1*еaiеi aсid) at a fixеd pН valuе thе сonсеntгation ofphеnolatе ions that seеm
tЪ bе tьe most геaсtivе foгm with rеspeсt to thе suгfaсе is minimal.
Polyphenols (II) and (III) arе not desorbеd by watеr (pH l-6), alсohol, aсеtone,
and сhloгоioгm, whiсh pгovidеs еvidеnсе foг stгong bonding ofthе adsorbatе' Thе сhanges
obsеrvеd in thе еlеctroni. ,p..t,u allow onе to makе somе judgеmеnts on thе struсturе of
adsoгption сomplехes. It tuгns out that thе rеfleсtion spесtra foг сompounds on thе suгfacе
and aЬsoгption spесtгa in thе сasе of solutions diffег сonsidегably (Tablе 1). The spесtra
for оompounаs 1it1and (III) display an analogous bathoсhromic shift of thе maximum as a
гesult oi adsoгption in.сompaiison with thе molесulaг foгm in solution, whiсh gives
еvidenсе for thе faсt that thеЪесhanism of bonding of poiyphenols with thе siliсa suгГaсе
is similar.
Tаblе 1
Spесtгal сhaгaсtеristiсs of adsoгbatе and аdsorptiоn сomplеxes
on suгfaсеCom In solution
Inеrt solvеttt
Aлthraquinonе
Alizaгin
yс=o 1680 сm_
)"o-o. З24 nm
i"-"'324 nm,334 nm
A yс=o 10 сm.
Ь}'o-,t 1О nm
)n*3З4 nrrl
Ь*437 nm406 nm, 424 nm
Alizaгin (pK' 5.5)
Quегсеtin ФK" 7 .5)
LI/а tе r -е thаno l m i хtu r е (1.. 1
},,* 4з5 nm (molесulе)
Ь* 5з0 nm (anion)
7.*3З6 nm
Ьo' 5ОО nm
Ь*385nm
304
оlutions of
;е stгength
эiесuiеs in
lf a similaг
.molесular
..се alizaгin
iесulaг and
ilantially, it
r and (III)
:е onsеt of
ээгotonatеd
:эr bonding
.:еd arе low
:r еn in thе
аdsoгption
:сsеnсе ofa
: that with
:..l еffесt of
:,::dегs thе
..:'э сasе of
:-s that sееm
j]-. aоеtonе'
Т:-е сhanges
:::uсturе of
r::lе suгfaсe
l:lе speсtra
шl'.jmum as a
..:.iоh givеs
; :a suгfaсе
----1-.:m
'i' : rm
Thе irгеvеrsibility of adsorption and substantialсhangеs in thе еlесtгоrtiс spесtгa foг
thе adsorbеd statеs givе еvidеnсе foг сhemisoгption of pol1;phеnols on siliсa whiсh sееms
to pгocеed through foгmation of сhеlatе гings. Whеn thе сooгdination numbег of siliсon
inсгeasеs up to six, thе abilitу foг сhеlating bеcomеs гathег геmaгkablе and may bе
сonsidегеd a spесifiс pгopегty of o-diphеnols [5] in whosе mоlесulеs thе oxуgеn atoms aге
at a distanсе eХaсtly equivalent to that геquiгеd bу thе struсtuге with oсtahеdral
сooгdination of siliсon atoms [6]. It is this possibility of fогming stгong сhеlates that is
геsponsiblе foг thе iггеvегsiblе сhaгaсtег оГ thе сhеmisoгption of сompounds (II) and (IIi)
whosе molесulеs havе thе nесеssary геaсtivе sitеs. one morе aгgument foг thе va1idity oi
this infегenсе is thе order in whiсh thе 2-* valuеs inсгеasе in thе сasе of tгansition n3o'
displaуеd in thе speсtгa foг alizarin in solution at diffеrеnt pH and on thе Sioz suгfaсе:
solvеnt, pI{ > з
Ь*= 535 nm
It is in this sequеnсe of thе struсtuгеs that thе еnеrgy of thе oгbital of n-еleсtгons foг
groups oН of phеnols maу inсrеasе' with the sаmе bеing also truе foг thе bathoсhгoпriс
shift of thе сoггesponding transition. It should bе еmphasizеd, howеvеr, that the struсturе
of thе adsогption сomplеx with oсtahеdral сoогdination оf siliсon atoms may bе сonsidеrеd
only as a limit struсtuге. With allowanсе foг stеriс hindгanсеs, formation of suгГaсе
сompounds of pоlyphеnols rr,hosе siliсon сooгdination numbег doеs not ехсеed fivе sееms
to bе moге pгobablе.
Thus, it has bееn establishеd that thе natuге ofa solvеnt ехегts a profound еffесt оn
thе mесhanism of foгmation and оn thе struоtuге оf adsoгption сomрlехеs on hydratеd
suгfaсеs of dispегsеd siliсas. In thе сasе of inегt solvеnts thе сornpounds studiеd aге
геvегsibly adsoгbеd thrоugh foгmation of hydгogеn bonds diгесtly with oН gгoups oг
thгough thе intегmediary of watег molесulеs in thе layег of sоlvatеs on thе suгГaсе fn thе
situation with polaг pгotoaсtivе solvеnts (watег, alсohol) thе adsoгption of mоlесular
foгms of natuгal polуphеnols on siliсa is nеgligiblе beсausе solvatiоn еffесts pгedominatе'
Thе adsoгption is obsегvеd only for dеpгotonatеd molесulеs оf polyphеnols in thе гangе of
pн > 3. It is an iпеvегsiblе pгoсеss, whiсh сan bе ехplainеd bу foгmation of suгflaсе
structuгes with stгong сooгdination bonds among siliсon atoms and adsorbate anions. In
pгaсtiсе it mеans that formation of сurative сompositеs on thе basis of siliсa entегosoгbеnt
and bioaсtivе polyphenols shouid bе еfrесtеd with cbligatory and duе aссount of the natuге
of ехtraсtants.
As is known [7], pгopепiеs of a сuгativе сompositе depеnd not onlу on its
сomposition but also on thе typе of its dosagе foгm, Composites Phуtosil сan be
administeгеd in the foгm of powdеrs, suspеnsions, tablеts, ointmеnts, еtс. In stomatоlogy,
foг instanсe, tгratmеnt Гoг vaгious disеasеs of oral сavity with Phцоsil is most еrесtiue
whеn this сompositе is in thе Гoгm of slow-disintеgгating tablеts, ouг ехpегimеnts havе
shown that tablеting of miхturеs of сomminutеd vеgеtal геsourсеs and highly dispeгsеd
siliсa makеs it possiblе to сrеatе соmpositеs whose hеaling proрегtiеs сan bе .u.n Ъigh..
than thosе of simple mесhaniсal miхtuгеs of powdегs oГ both substanсеs. This synегgistiс
еffесt is pгobably due to thе faсt that duгing the сoursе of pгеssing thеге arise interaсtiоns
among paгtiсlеs of siliсa and vеgеtal biopolуmеrs. Formation of suсh usеful adduсts rnaу
vary thе pгеpaгation adsoгption timе oг affесt thе pегmеability oГ сеll mеmbranеs theгеby
inсreasing the bioaссеssibility of aсtivе сompounds. Thе intегaоtions among paгtiсlеs of
adsoгbеnts and biopolуmегs of plant tissuеs should affeсt first of all thе сhaгaсtегistiсs of
laуегs оf watег bound to thе suгfaсе, as it has bеen observеd in thе сasе of intегaсtions
bеtwееn thе dispeгse siliсa suгfaсe and protеin moleсulеs dissolvеd in *,atег [8] Suсh
intегaсtions сan bе studied by tесhniquеs of high геsolution ргoton magnеtiс геsоnanсе
solvent, pн < з
Ь*= 4З5 nm
suгfaсе
Ь*= 5ОО nm
з05
spеctгosсopу in сonjunсtion with thе proсеdurе that involvеs frеezing of a liquid phasе [9].
iьl. upp.йh pегmits onе not onlу io dеtегmine thе amоunt of bound watеr but also to
.aке еitimation of a hуdratе .ou.i in tегms of thе intегactions with thе suгГacе as wеll as
to сalсulatе valuеs oгй. suгГaсе еnегgу of adsorbеnts on thе basis of the total dесreasе in
thе enеrgy ofa systеm сausеd bу the prеsеnсе ofthе phasе intегГaсе [10].
iЬprуing thе нl NМR mеthЬd wе havе invеstigatеd hуdгation proсеssеS on thе
suгГaсе of dispегsеd siliсas, сomminutеd mеdiсinal plants (matгiсary, sagе)' and thеir
p,",,"а mixtuтеs Driеd plants wеrе сomminutеd to рaгticlе sizеs of l_15 рm, mixеd in a
гatio of i:5 with dispеrsеd siliоa (с/ < 0.01 mm), and tablеtеd a1 a pгеssuге of 15хl06 Pa
Thе expегimеntal and сomputational pгoсеdures aге dеsсribеd in Rеfs' [s-10]. Thе rеsults
of ou. inu.stigation of thе йydration proсеssеs arе summaгizеd in Тablе 2, whеrе AG is thе
frеееnеrgyсhangе,Сn.oi,thеamountofboundWatеr,sil,М'andSstandforsiliсa'
matгiсary'andsagегеspесtivеlу.Upрегindiсеswandsdеnotеwеaklуandstronglуbound
watеr; lowеr indеxp is usеd to dеnotе pгеssеd samplеs'
Fгom thе data.listеd in Tablе j, it гollo*, that in thе сasе of pгеssеd сoщpositеs
onе сan obsегvе an inсгеase in thе frее suгfaсе еnеrgу. In thе sуstеm with matгiсary this
inсгеasе (almost by a faсtor of two) is attainеd owing to a гisе in thе сonсеntгation of both
;;;йly uno *"uкtу bound watег, whilе in thе situation with sagе a гisе is on1у obsегvеd
for thе сonсеntration of wеakly bound watеr. on thе basis of thе геsults aсhievеd onе сan
infег that the proсеss of pгеssing thе Ph1tosil соmpositеs doеs bгing abоut intеraсtions
;;;"g paпiсlеЪ of si1iсa unа ptuni, and thш thеsе intегaсtions lеad to somе сhangеs in thе
struсtuге оf hуdгatе сovегs оirеa"ting surfaсеs. Fuгthегmorе, thе intегaсtions bеtwееn thе
siliсa suгГaсе and pгotеin molесulеs is a".o.puniеd with dеhydгation of thе suгfaсе [8],
whilе in thе сasе of thе intегaсtions among
'oiiа
paгtiсlеs thе opрositе is truе, namеlу thе
dеgrеe of hydгation of the suгfaсе inсrеasеs' ln allpгobability, adduсts in suсh sуstеms arе
formеd duе to thе aоtion of еlесtrostatiс foгсеs bесause thе prеsеnсe of wеaklу bound
Watеr сan alsо lеad to an inсrеasе in thе dеgгее of hydration, i.e, thе еffесt is a long-гangе
onе.
Еarliег it was shown [10] that thе main сause of inоreases in thе dеgrее of
hуdгation of suгГaсе in watег suspеnsions was foгmation of unlikеlу сhargеd poгtions of
the suгГaсе and thеir intегaсtion witь dipotеs of watег molесulеs. Thе intегaсtion of this
typе is a long-гangе onе b"сuu," it is еffесtivе at distanсеs сompaгablе with thе basis of
surfaсе dipoles (intегval bеtwееn сhaгgеd poгtions. of thе '.YР..l
In systеms undеr
сonsideration thе appeaгanсе of сhaгgеs on ih. suгГaсе of solid paгtiсlеs сan bе сausеd
еithеr by гeaсtions oipioio" transfеr йom.hydroхyl groups on thе siliсa suгГaсе to pгoton-
aссepting (eleсtгon.JJnating) sitеs on molесulеs ЬгЪiopotуmeгs oг by polarization of thе
suгfaсе poгtions ut point, oi.to,. сontaсt bеtwееn paгtiсlеs of siliсa and сuгativе plants. In
any сasе, suсh a .to,. .ontu.t should lеad to dеformations of сell mеmbгanеs, to inсrеasе
in thеir pегmеabi1itу, and, subsеquentlу, to ехtеnsion of biоaссеssibility of aоtivе moleсulеs
ltll
on thе basis of thе data availablе in litегatuге it is possiblе to draw somе tеntatlvе
infеrеnсеs about сoпеiation bеtwееn the сhemiсal structurе of biomolесulеs and thеiг statе
in solution, on the on. ьunа, and thе meсhanism of thеir adsoгption, on thе othег hand. A
сonsidеrable сontribution to adsorption on siliсa from aqueous solutions of biosubstanсеs
(paпiсulaгlу oг to*-motесulaг subЪtanсеs) is pгobably madе by
.еleсtrostatiс
intегaсtions'
At physiologiсal pH valuеs of solutions whеn thе siliсa surfaсе is charged negatively and
low-molесulaг biosubstanсеs in thеsе solutions arе in thе foгm of anions, thеiг molесulеs
arе not adsorbеd or aге adsoгbеd only slightlу. Flесtгostatiс interaсtions makе also а
сonsidеrablе сontгibution to adsorption oгЪiopotymегs (prot.еins, nuсlеiс aсids). Polaг
biomolесulеs tгo. "*u'pre
gluсosamine) сan bЪ sorbеd on siliсa thгоugh foгmation оf
306
hydгogеn bonds with.silanol gгоups on thе siliсa suгfaсе. Thе adsoгptlon is markеdlyеnhanсеd duе to thе faсt that on a biopolymег moleсulе thеrе aгe many sitеs сapablе offoгming hydrogеn Ьonds with thе Sio2
'uй.. (this seеms to be the сasе fo. gluсosaminеglyсanеs). Biomolесulеs that ехist in solution in thе foгm of zwittегions (tесithin,pyгodoxinе) сan bе soгbеd at thе ехpensе of intегaсtions bеtweеn nеgativе sitеs оn thеsiliсa suгfaсе and positive sitеs on thе Ьiomolесulеs.
Тhе mесhanism, of
.adsoгption of pгotеins is still undеr disсussion. Thе bеst-rеasonеd sееms to be the viеwpoint a.ссoгding to whiсh thеге aге two u]timatе typеs ofpгotеins diffегing in thе mесhanism of thеiг aЬsorption on a solid ,uru".' namely .haгd,
and .soft' pгoteins. Adsoтption of .haгd' protеins who'. .ot..ui., aге distinguishеd foгthеir гigid adhегеnсе (foг ехamplе гibЬnuсlеasе) i, gou",n.J b! etесtгostatiс aпdhydгophobiс intегaсtions as wеll ui ьу d.ьyd.ation of thе suгfaсе and pгоtеin molесulеs.During adsогption of .sоft' pгotеins ih... Ъpp.u,s an additiоnal dгivirrg forсе bound upwith сhangеs in thе struсtuге of pгоtеin
'ot..ut.,, and thе сontгibution madе by this foгсеmaу ехсееd unfavourablе сontгibutions made bу еleсtrostatic .;;;йl"" As a result, inсontгast to.hard'pгotеins, .soft,pгotеins
сan bе adsoгbеd ьу.a ьi,сгophiliс similarlyсhaгgеd suгfaсе'
TаbIе 2
Еffесt of pгеssing on hydгation сharaсtегistiсs of сompositе Phltosil"
Systеm
(-s
"H:o
mg g-r
c"ro'
Мg g-,
^r:
r
КJ mol
^cw
КJ mol
tGt
kJ mol-r
phasе [9].
rut also to
as wеll as
jесrеasе in
sеs on thе
and thеir
niхеd in a
.5х i06 Pa-
rhе rеsults
э -\G is thе
' ior siliсa'
:.*:lv bоund
;опrpositеs
'::lсary this
.:: ofboth
' оbsеrvеd
3] onе сan
::.:еraсtions
;.3еs in thе
:::.лееn thе
:-зaсe [8],
..molrl fhр
i. siеms aге
''-...', bound
' ..:.]g-rangе
, j.оrдр nf
: - гtions of
т : of this
:. .эasis of
i:_'s undеr
I .3 сaused
t r. Droton-
aг ..: of thе
иt :.ants. In
'
. . .nс'гeasе
в-lэlесulеs
Е: '.ntativе
ш .еiг statе
Aс. rand. A
k. -]stanсеs
iт' : :3сtions'
р ' еly and
Е * эlесulеs
Ь.. also a
l. -. ' Polaг
p* :rion of
I
I
i
М
s
sil
silP
500
400
250
2'/0
700
.t zu
J .,/,
3.0
4.5
28
40
4.0
14.40
1З 40
t2.80
858
30.60
17.70
400
2s0
130
б00
580
0.8
1.2
l.J
1.0
t.2
(Sil+М)o
(Sil+S)o
As геgaгds thе notation, sее thе tехt
TнЕ RЕsULTs oF CLIN'IсAL TRIALS oF PIIYTOSIL
Wе сonstruсtеd Ph1tosil tablеts of mеdiсinal plants гiсhеst on сomрonеnts, whiсhhavе anti - inflammatory and immunе modulativе aсti;itу тhе ethonf was addеd to tablеtsfor thе pгophуlaxis of thе dеvеloprnеnt of baсtегial сoйрliсations. As tье apр1iсation oftablеts is сonvеniеnt at thегapy of disеasеs of mouth сaviiy fiгst of allwе tеstеd Phytosilinthе stomatology' Thе tеsts wегe сaггiеd out on Faсulty of thегapеutiсal stomatology oгNational mеdiсal univегsity named by o'o'Bogomolеtс (doсtor of mеdiсal sсiеnсеsЕ.S,Yavoгska, сandidatе of mеdiсal iсiеnсеs AV'Vidегska) and Faсultу of сhildгеnstomatology of Kiеv mеdiсal aсadеmy of postgгaduate еduсation (сandidatе of mеdiсalsсiеnсеs H.o Savitсhuk, aspiгant S V.Nеdaйkovska).
It is еstablishеd, []1] that сomposite Phytosil is еffесtivе at loсal appliсation astablеts wiсh slowlу геsolvе in a сavitу of a mouth. Thе prеpaгаtion has inсгеasеdpathogеnеtiс pгopегties, It was maгkеj thе геmoval of pain' signifiсant dесrеasе ofsymрtoms of thе muсous shell Inflammation (oеdеma, ьуpoiе';u; ina гu,. еpithеlization
З01
of еrosions and ехсoгiatings. At thе patiеnts, whiсh suffеr glossitis thе геduction of oеdema
of soft fabгics and muсйs shеll of a сavity of a mouth, rеduсtion of fuг and, main,
еlimination of paгеsthеsia wеrе obsегvеd. Еspесialiу еffесtivе thеrе weге Phytosil tablеts in
the trеatmеnt of сhildгеn having aсutе heгpеs stomatitis (Tablе 3).
Thе analysis of thesе rеsults has геvеalеd highеr сliniсal еffiсiеnсу of Phytosil
tablеts rather of Еtiotгopiс tгеatmеnt and Lеvamizol bу all invеstigated сriteria. Thе сliniсal
еfiiоiеnсу of Phytosil сЪ.positе сoпеsponds to a levеl of thymic immunomodulatoгs, but
ihе appliсation of phyo"ompositе simplifiеs and makеs сhеapег mеdica1proсess.
Naturallу,
.in-tlrе
givЪn timе nЬt all phaгmaоologiсal propeсiеs of a nеw сlass of
mediсinal pгеparations oifamily Phfosil a,i kno*n for us' Thе fuгthег physiсo.сhеmiсal
and mеdiсal-biologiсal ,",.u,.h., willbе diгесtеd on еxpansion of a sресtrum of^aсtion of
pгeрaгations havе bееn devеlopеd alrеadу and сrеation of nеw сompositеs, effесtivе. in
it,.iupу and prеvеntive mainiеnanсе of оardio-vasсulaг, gastгiс-intеstinal and other
diseasеs'
Tаblе 3
.;;;"ipiсturе of effiсiеnсу of rеgionalimmunothеrapу of childгen's aсutе hегpеs
stomaтltls
Time of trеatmеnt (daуs)
Сliniсal paramеtеrs Waу of tгеatmеnt rathеr hеavy
foгm of
disеasе
heavy form
of diseasе
Еpithelization (rеstoгation)
of elemеnts of a damage
Phyosil
Vilosеn
Thymalin
Lеvamizol
Еtiotropiс tгеatmеnt
anэ,l
з.,7
3.8
5.0
56
4.з
5.0
4.0
5.8
6.1
Disappеaranсе ofthе
Gingivitis
(inflammatiоn of thе gum
Tissuеs)
Phytosil
Vilosеn
Thymalin
Lеvamizol
Еtiotгopic trеatmеnt
5.0
5.1
5.5
6.6
I.>
5.5
5.8
6.5
I.+
9.0
Disappеaranсе of thе
Lуmphadеnitis
(inflammation
of lymph nodеs)
Phytosil
Vilosen
Thуmalin
Lеvamizol
Еtiotгopiо tгеatmеnt
6.9
7.0
9.6
10.8
,ll я
'7.9
8.0
10.7
11.8
15.1
in the hearу foгm) and way of trеatmеnt:
29(1,. Phytosil, 24(ll) . Vilosеn, 24(10) - Thymalin, 24(10) - Levamizol,
з3(17) . Еtiotгopiс trеatment
308
f oеdеma
rd, main,
tablеts in
'Phytosil
rе сliniсal
atoгs, but
r сlass of
l.сhеmiсal
: aсtion of
fесtivе in
ld othеr
г: (days)
:.еalу foгm
:t disеasе
4.з
5.0
4.0
5.8
6.1
5.5
5.8
6.5
l.ч
9.0
8.0
10.7
I1.8
151
li
tЕ
Aсkпowlеdgеmеnts
Thе authoгs thank Doсtoг of Меdiсal Sсiеnсеs, pгоfеssor Е.Gjulling fbг thе hеlp in
dеveloрmеnt of a сompositе tЪгmula' сhoiсe of a diгесtion and гealization of сliniсal tеsts.
Refеrеnсеs
1 Chuiko A A , Pеntуuk o o ' Sсiеntifiс Pгinсiplеs of Devеlopment of Меdiсinal
Pгеpaгations оn thе basis of Highly Dispeгse Siliсa// Pгoс' of thе Sсiеntifir Чession oГ
thе.Dеpaпmеnt of Chеmistry оf thе NAS of Ukrainе Dеdiсatеd to the 80ш Annivегsary
of the NAS of Ukгainе' Kharkiv, Junе 9-l1, 1998; Book Abstг. pp. 36-51- (in
Russian).
2. Georgiеvskii V'P., Komisarеnko N F , Dmitruk s.Е', Biologiсallу Aсtivе Substanсes
of Mеdiсinal Plants. Novosibirsk: Nauka, 199О.. ЗЗ2 p.
3. Chuiko A.A., Goгlov Yu.I. Suгfaсe Сhеmistry of Silica: Suгfaсе Struсtuге, Aсtivе
Sitеs, Soгption Месhanisms' Kyiv: Naukova Dumka, 1'992. - 248 p. (in Russian).
4. Koиyukhova N.I , Pogoгеlyi V.K. Thе adsorption of bioactivе polуphеnols on siliсa
oxidе from inегt solvеnts ll ,rJkr
Khim. Zh.. |997. - V,63, N 1.2.. P,2О-25' (in
Russian).
5. Ilег R.K., Thе Chеmistry of Siliсa: Solubility, Polymеrization, Colloid and Suгfaсе
Propеrtiеs, and Bioсhеmistry Nеw York - Chiсhеsteг - Brisbanе - Toronto: A Wilеy-
lntегsсiеnсе Publiсation, I979. _ 1|27 p,
ll
Тentsova A.I. (Еd.), Phaгmaсist Rеfеrеnсе Book, Меditsina, Мosсow, 1981 - 354 p
(in Russian)
Gеorgiеvskii V'P.' Konеv F A.(Еds), Tесhnology and Standaгdization of Меdiсinal
Substances. Khaг'kov: ,.RIRЕG'', 1996. _ 784 p. (in Russian).
Tuгov V'V., Barvinсhenko V'N. Struсtuгally огdеrеd suгfaсе laуеrs of watеr at thе
Sioz/iое intегfaсе and in{luеnсе of adsoгbеd molесulеs of pгotеin hуdгolysatе on
them/l Colloids and Suгf. B. - 199.7 ' - N' 8 -P. 125-1з2'
Studу of intеraсtion of albuminе serum йth dymеthуlsulfoxidе on PМR spесtгa of
thе fгozеn watеr solutions / v'K. Pogorelyi, v.v' Tuгov, A.v. Tuгov,
V.N.Baгvinсhenko// Tеoг.Bksp.Khim.- 1989,- v. 25,N l.. P.l04-108. (in Russian).
Turov V.V., Lеboda R' Appliсation of Hl NМR spесtrosсoрy mеthod for
dеtегmination of haгaсtегistiсs of thiп layer of wateг adsoгbеd on thе suгfaое of
dispегsеd and porous adsoгbеnts /i Colloid and Intегface Sсi.. 1999 - У.79. -
Р.17з-2|1.
Thе invеstigation of physiсo.сhemiсal and сliniсai propегtiеs of mеdiсinе сompositеs
bеlonging to thе Ph}tosil familу/ A.A. Chuiko, V.K Pogorеlyi, V.N Baгvinсhenko,
N.A'Lipkovska, N.I.Koи1ukhova, V v Turov ll Visnyk Vinnуts'kogo
Dеrzhavnogo Меdyсhnogo Univегsреtu'. l999. - N 3.. P ' 253.256, (in Ukrainian).
n
8.
o
10.
309
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| id | oai:ojs.pkp.sfu.ca:article-69 |
| institution | Surface |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-22T19:30:03Z |
| publishDate | 2001 |
| publisher | Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine |
| record_format | ojs |
| resource_txt_mv | surfacezbircomua/31/a4f618cde07b671d4c9664ce7789fe31.pdf |
| spelling | oai:ojs.pkp.sfu.ca:article-692018-11-27T09:42:39Z Adsorption of bioactive molecules of medicinal plants Adsorption of bioactive molecules of medicinal plants Adsorption of bioactive molecules of medicinal plants Pogorelyi, V. K. Barvinchenko, V. N. Lipkovska, N. A. Turov, V. V. Chuiko, A. A. The adsorption of some herbal bioactive polyphenolics (anthraquinones, flavons) from solutions of different polarity onto a surface of highly dispersed silica (entherosorbent Silics) is studied. It is found that the structure of adsorption complexes and the mechanism of their formation depend essentially on the solvent nature. Preliminary clinical researches have shown high medecinal activity of Phytosil composites regarding children’s herpes stomatitis. The effectiveness of Phytosil composite corresponds to the level of thymic immunostimulators, but its application makes the process of treatment simpler and cheaper. The adsorption of some herbal bioactive polyphenolics (anthraquinones, flavons) from solutions of different polarity onto a surface of highly dispersed silica (entherosorbent Silics) is studied. It is found that the structure of adsorption complexes and the mechanism of their formation depend essentially on the solvent nature. Preliminary clinical researches have shown high medecinal activity of Phytosil composites regarding children’s herpes stomatitis. The effectiveness of Phytosil composite corresponds to the level of thymic immunostimulators, but its application makes the process of treatment simpler and cheaper. The adsorption of some herbal bioactive polyphenolics (anthraquinones, flavons) from solutions of different polarity onto a surface of highly dispersed silica (entherosorbent Silics) is studied. It is found that the structure of adsorption complexes and the mechanism of their formation depend essentially on the solvent nature. Preliminary clinical researches have shown high medecinal activity of Phytosil composites regarding children’s herpes stomatitis. The effectiveness of Phytosil composite corresponds to the level of thymic immunostimulators, but its application makes the process of treatment simpler and cheaper. Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2001-06-10 Article Article application/pdf https://surfacezbir.com.ua/index.php/surface/article/view/69 Surface; No. 4-6 (2001): Chemistry, Physics and Technology of Surface; 301-309 Поверхность; № 4-6 (2001): Химия, физика и технология поверхности; 301-309 Поверхня; № 4-6 (2001): Хімія, фізика та технологія поверхні; 301-309 3154-8091 3154-8083 en https://surfacezbir.com.ua/index.php/surface/article/view/69/68 Авторське право (c) 2001 V.K. Pogorelyi, V.N. Barvinchenko, N.A. Lipkovska, V.V. Turov, A.A Chuiko |
| spellingShingle | Pogorelyi, V. K. Barvinchenko, V. N. Lipkovska, N. A. Turov, V. V. Chuiko, A. A. Adsorption of bioactive molecules of medicinal plants |
| title | Adsorption of bioactive molecules of medicinal plants |
| title_alt | Adsorption of bioactive molecules of medicinal plants Adsorption of bioactive molecules of medicinal plants |
| title_full | Adsorption of bioactive molecules of medicinal plants |
| title_fullStr | Adsorption of bioactive molecules of medicinal plants |
| title_full_unstemmed | Adsorption of bioactive molecules of medicinal plants |
| title_short | Adsorption of bioactive molecules of medicinal plants |
| title_sort | adsorption of bioactive molecules of medicinal plants |
| url | https://surfacezbir.com.ua/index.php/surface/article/view/69 |
| work_keys_str_mv | AT pogorelyivk adsorptionofbioactivemoleculesofmedicinalplants AT barvinchenkovn adsorptionofbioactivemoleculesofmedicinalplants AT lipkovskana adsorptionofbioactivemoleculesofmedicinalplants AT turovvv adsorptionofbioactivemoleculesofmedicinalplants AT chuikoaa adsorptionofbioactivemoleculesofmedicinalplants |