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Криозащитные свойства растворов на основе непроникающего ОЭГ n=25 в комбинации c проникающими криопротекторами при замораживании эритроцитов человека

Криозащитные свойства растворов на основе непроникающего ОЭГ n=25 в комбинации c проникающими криопротекторами при замораживании эритроцитов человека

Cryoprotective efficiency of сryopreservatives, based on combination of extracelular cryoprotectant oxyethylated glycerol with polymerization degree n = 25 (OEGn=25) with penetrating cryoprotectants 1,2-propanediol (1,2-PD), dimethyl sulphoxide (DMSO) and dimethyl acetamide (DMAc) during red blood...

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Bibliographic Details
Main Authors: Pakhomova, Yuliya S., Chekanova, Valentina V., Kompaniets, Antonina M.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
Subjects:
еритроцити
кріоконсервування
комбіновані кріозахисні середовища
оксиетильований гліцерин
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/7
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Summary:Cryoprotective efficiency of сryopreservatives, based on combination of extracelular cryoprotectant oxyethylated glycerol with polymerization degree n = 25 (OEGn=25) with penetrating cryoprotectants 1,2-propanediol (1,2-PD), dimethyl sulphoxide (DMSO) and dimethyl acetamide (DMAc) during red blood cells freezing was studied. Freeze-thawing in solutions of OEGn=25 and 1,2-PD, DMSO and DMAc mixed in 5:1 ratio, as well as with DMAc in ratios of 2:1 and 1:1 resulted in the reduction of osmotic fragility of red blood cells comparing to non-treated cells. The highest level of post-thaw survival of red blood cells according to indices of hemolysis and content of free hemoglobin was found for 30% OEGn=25 solution. Probl Cryobiol Cryomed 2013; 23(1):26-39.

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