Взаємодія дезоксирибонуклеїнової кислоти з поверхнею модифікованого аміносиланом силохрому

• Datum: 2025
• Hauptverfasser: Markitan, O. V., Kozakevych, R. B., Kamenskyh, D. S., Tertykh, V. A.
• Format: Artikel
• Sprache: Ukrainisch
• Veröffentlicht: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2025
• Schlagworte: силохром; хімічне модифікування; дезоксирибонуклеїнова кислота; адсорбція; ізотерма; моделі адсорбції
• Online Zugang: https://www.cpts.com.ua/index.php/cpts/article/view/772
• Назва журналу: Chemistry, Physics and Technology of Surface

Materials capable of sorbing nucleic acids are in great demand for the development of sensor systems for biological purposes, analytical separation, and gene delivery. Due to their unique properties, nanoscale oxide materials are increasingly becoming the basis of research and development of new catalytic and sensor systems. Silicas are of particular interest. The stability of their physicochemical parameters, significant rate of reaching equilibrium, large surface areas, and mechanical stability contribute to the wide use of these materials for modification and immobilization on their surface various functional groups. Chemical modification of the silica surface allows one to control the strength of the adsorption interaction of substances with the surface, preserving the functionality of the adsorbate, ensuring the reversibility of the process and increasing the selectivity of materials.The purpose of this work was to study the adsorption of deoxyribonucleic acid from an aqueous solution on the surface of silochrome modified with 3-aminopropyltriethoxysilane, depending on the pH of the medium and the concentration of the adsorbate. It has been shown that the driving force of adsorption is the electrostatic interaction between positively charged aminopropyl groups of the sorbent surface and nucleic acid anions. Adsorption of DNA in the acidic region can be carried out due to dispersion forces or hydrogen bonds between surface functional groups and highly polar secondary phosphate groups on the outer surface of the nucleic acid molecule. Based on the calculated thermodynamic parameters, the process of interaction of deoxyribonucleic acid with the surface of aminosilane modified silochrome is believed to be spontaneous and proceeds according to the ion-exchange mechanism, as a result of which sorbate molecules bind to the surface quite strongly. The implementation of electrostatic interaction between aminosylochrome and deoxyribonucleic acid allows to stabilize its structure in a certain way, creating the possibility of further interaction by intercalation with biologically active substances. This will allow the use of such organo-mineral systems as biocompatible carriers and as model structures in biotechnological, medical, and catalytic research.