Коррекция популяций ценобионтов Bifidobacterium spp. и Lactobacillus spp. у мышей с экспериментальным дисбиозом кишечника после терапии иммобилизованными на энтеросорбентах пробиотиками, хранившимися при –80 и –196°С

• Дата: 2015
• Автори: Vysekantsev, Igor P., Babinets, Olga M., Martsenyuk, Valentina F., Shatilova, Larisa Ye., Scheglov, Andrey V., Voyda, Yulia V.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2015
• Теми: низькотемпературне зберігання; іммобілізовані пробіотики; ентерособенти; дисбіоз кишечника; ценобіонти; сорбенти; миші
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/791
• Назва журналу: Problems of Cryobiology and Cryomedicine

The research revealed the recovery of the Bifidobacterium spp. and Lactobacillus spp. cenobiont populations in mucin of mice with experimental intestinal dysbiosis after treatment with free and immobilized on enterosorbents probiotics, stored for a year at a temperature of –80, –196°C. Experimental intestinal dysbiosis was simulated by Ampicillin and Metronidazole oral administration. Mice with induced dysbiosis were treated with free cells of S. boulardii, B. bifidum, L. bulgaricus probiotics, the mixtures of free cells with enterosorbents as well as the complexes of probiotic cells immobilized on enterosorbents. The enterosorbents based on activated carbon Sorbex and SCMS-1 were used. Immobilized probiotics have been found to provide a more rapid and complete recovery of cenobiont populations. Low-temperature storage for a year (observation period) and temperature storage regimen did not affect the therapeutic properties of free and immobilized cells of probiotics. The mechanisms of action of immobilized probiotics are under discussion.Probl Cryobiol Cryomed 2015; 25(3):267-286