Влияние автоклавированных экстрактов плаценты человека на фазовое поведение суспензий клеток при температуре ниже 0°С

• Видавець: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
• Дата: 2013
• Автори: Zinchenko, Aleksandra V., Bobrova, Elena N., Schetinsky, Miroslav I., Govorova, Yulianna S.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
• Теми: фазовые переходы; дифференциальный сканирующий калориметр; экстракт плаценты; автоклавирование; суспензии клеток; СПЭВ; эритроциты; Candida albicans; фазові переходи; диференційний скануючий калориметр; екстракт плаценти; автоклавування; суспензії клітин; СПЕВ; еритроцити; phase transitions; differential scanning calorimeter; placenta extract; autoclaving; cell suspensions; SPEV; erythrocytes; Candida albicans.
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/8

Phase transitions in human placental extracts during cooling at -196°C and the effect of fresh and autoclaved extracts on phase transitions in cell suspensions were studied. It has been found that after cooling with the rate of 3.3(3)deg/s in cell suspensions or suspending media the process of crystallization termination developed at the stage of heating with the rate of 8.3(3)x10-3 deg/s. After dilution of the suspension of Candida albicans and SPEV cells with autoclaved extract this process was not observed. It was found that the crystallization of eutectic mixtures was developed in frozen placental extracts unlike the mixture of extracts with erythrocytes and C. albicans cells. Probl Cryobiol Cryomed 2013; 23(1):40-48.