Преципітація білків із холодоадаптованих личинок Tenebrio molitor у присутності ПЕГ-6000

• Date: 2015
• Main Authors: Gulevsky, Aleksandr K., Grischenkova, Elena O., Relina, Liana I., Tretyak, Diana V.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2015
• Subjects: холодова аклімація; білки; поліетиленгліколь; преципітація; електрофорез
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/801
• Journal Title: Problems of Cryobiology and Cryomedicine

Spectra of PEG-6000-precipitated proteins from cold-acclimated and non-acclimated Tenebrio molitor larvae were comparatively studied. The composition of precipitated proteins from cold-acclimated and non-acclimated T. molitor larvae was shown as differed qualitatively. In particular, the protein spectra of filtrates from cold-acclimated larvae had proteins with molecular weight of 11 kDa, which were absent in non-acclimated insects. There was observed a significant difference in precipitate content at polyethyleneglycol concentrations of 12% and higher during protein precipitation from homogenates of cold-acclimated and non-acclimated larvae. Proteins from the filtrates of cold-acclimated larvae in the presence of 6% PEG-6000 were precipitated by 44 and 56% greater, than those from non-acclimated species at 22 and 4°C, respectively. The increasing of PEG-6000 concentration from 4 to 6% resulted in appearance in the precipitates from cold-acclimated larvae of proteins with molecular-weight range of 30–42.5 kDa, which were absent in non-acclimated insects.Probl Cryobiol Cryomed 2015; 25(4):350-358.