Криоконсервирование ядросодержащих клеток кордовой крови под защитой непроникающего криопротектора ПЭО-1500

• Main Authors: Babijchuk, Lyubov A., Mykhailova, Olga A., Ryazantsev, Vladimir V., Zubov, Pavel M., Migunova, Regina K.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2016
• Subjects: гемопоетичні стовбурові клітини; ядровмісні клітини; кордова кров; кріоконсервування; непроникаючий кріопротектор; ПЕО-1500
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/813

Development of wash-free cryopreservation methods for cord blood nucleated cell fraction, including the population of hematopoietic stem/progenitor cells, is an actual task for their long-term storage. Present investigation involved the technique of cord blood nucleated cells cryopreservation, comprising the isolation of cell population by the original two-step centrifugation method, the treatment with non-penetrating cryoprotectant polyethylene oxide with molecular weight of 1500 (PEO-1500) and freezing by the two-step program. This technique enabled to preserve more than 72% CD45+ and 75% CD34+ cells with the viability indices of correspondingly 83 and 91% and higher. Herewith, an increased content of reactive oxygen species (42.7 ± 6.3%) on the background of high cell number and their viability may be considered as a cells’ physiological response to the effect of cryopreservation factors.Probl Cryobiol Cryomed 2016; 26(1):24-34.