Влияние пропиленгликоля и полиэтиленгликоля с молекулярной массой 1500 на микровязкость мембран эритроцитов

• Дата: 2016
• Автори: Nardid, Oleg A., Cherkashina, Yana O., Ivanov, Leonid V., Nardid, Eduard O., Lyapunov, Aleksandr N., Mamontov, Vladimir V.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2016
• Теми: криопротекторы; эритроциты; мембраны; микровязкость; электронный парамагнитный резонанс; спиновые зонды; cryoprotectants; erythrocytes; membranes; microviscosity; electron paramagnetic resonance; spin probes; кріопротектори; еритроцити; мембрани; мікров’язкість; електронний парамагнітний резонанс; спінові зонди
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/814
• Назва журналу: Problems of Cryobiology and Cryomedicine

Electron paramagnetic resonance of spin probes was used to study the effect of low and high molecular weight CPAs on microviscosity of erythrocyte membranes. Research data on rotational mobility of the fatty acid spin probes in erythrocyte membranes after 5 min incubation in 15 % propylene glycol solution indicate the absence of strong changes in membrane microviscosity. Introduced polyethylene glycol with m. w. 1500 (PEG-1500) at a final concentration of 15% has been shown to increase the membrane microviscosity at 60 min exposure. This is explained by the fact that the molecules of PEG-1500 do not pass inside the cells and are localized on the membrane surface to cause immobilization and inhibiting the rotational mobility of the spin probe. In the initial period of time under these concentrations of PEG-1500 the injury of liquid crystal membrane structure and disorder of the protein-lipid interactions in the membrane prevail over the dehydration of cells.Probl Cryobiol Cryomed 2016; 26(1):35-44.