Особенности витрификации мезенхимальных стромальных клеток в составе альгинатных микросфер

• Дата: 2024
• Автори: Zaykov, V. S., Petrenko, Yu. A., Trufanova, N. A., Pravdyuk, A. I., Petrenko, A. Yu.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2024
• Теми: мезенхімальні стромальні клітини; альгінатнi мікросфери; вітрифікація; мультикомпонентний розчин кріопротекторів; ступінчасте додавання кріопротекторів
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/93
• Назва журналу: Problems of Cryobiology and Cryomedicine

Cryopreservation of cells encapsulated in alginate microbeads is one of the important issues of tissue engineering. The present study was directed on the estimation of the effect caused by of duration of alginate encapsulated mesenchymal stromal cells (MSCs) exposure in multicomponent cryoprotective solution DEPS-1 (10% DMSO, 20% EG, 20% 1,2-PD and 0.5 M sucrose) on survival and metabolic activity after vitrification and rewarming. It was shown using of multicomponent cryoprotective solution and stepwise exposure allowed to achieve successful cryopreservation by means of vitrofication of MSCs encapsulated in alginate microspheres. The main difference between vitrification of MSCs encapsulated in microspheres vs. single cells was the longer exposure with CPA.