Влияние условий удаления криопротектора ДМСО на жизнеспособность и колониеобразующую активность криоконсервированных клеток эмбриональной печени человека

• Date: 2002
• Main Authors: Skorobogatova, N. G., Grischuk, V. P., Petrenko, A. Yu.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2002
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/984
• Journal Title: Problems of Cryobiology and Cryomedicine

The authors studied the influence of various conditions of Me2SO cryoprotectant removal on viability and colony forming activity of freshly isolated and cryopreserved cells of human fetal liver (HFL) of 7-10 gestation weeks. Usage of Hanks solution as a washing-out medium (WM) leads to a decrease in...